caa a22 4500 465795153 CHVBK 20180323112035.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00308563 doi (NATIONALLICENCE)springer-10.1007/BF00308563 Induction of an ATP-polymerizing enzyme in TMV-infected tobacco and its homology to the human 2′-5′ a synthetase [Elektronische Daten] [Naamit Sher, Orit Edelbaum, Zehava Barak, Gideon Grafi, Yehuda Stram, J. Raber, Ilan Sela] Several reports have indicated that tobacco carries an enzyme (APE) that, in the presence of poly (rI):(rC), polymerizes ATP to oligoadenylates. This paper demonstrates that the tobacco APE system comprises several proteins (estimated sizes: 32, 42, 67, and 84±10% kD). Only one of these proteins (the "67-kD” form) binds to poly (rI):(rC). This APE form has been purified by affinity chromatography on a synthetic ds-RNA column. Four tobacco proteins, including the purified one, crossreact with antibodies against the human enzyme, 2′-5′ A synthetase. The ATP-binding capacity of some of these proteins has also been demonstrated. The amount of plant oligoadenylates obtained by polymerizing ATP with the purified APE form allows, for the first time, their direct analysis by TLC. The TLC analysis indicated that the oligomer produced by APE is not identical to the 2′-5′ oligoadenylate. Kluwer Academic Publishers, 1990 interferon nationallicence TMV nationallicence tobacco nationallicence 2-5 A synthetase nationallicence Sher Naamit Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut Edelbaum Orit Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut Barak Zehava Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut Grafi Gideon Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut Stram Yehuda Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut Raber J. Department of Virology, The Weizmann Institute of Science, Rehovot, Israel aut Sela Ilan Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut Virus Genes Kluwer Academic Publishers 4/1(1990-06-01), 27-39 0920-8569 4:1<27 1990 4 11262 https://doi.org/10.1007/BF00308563 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00308563 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sher Naamit Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Edelbaum Orit Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Barak Zehava Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Grafi Gideon Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Stram Yehuda Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Raber J. Department of Virology, The Weizmann Institute of Science, Rehovot, Israel aut NATIONALLICENCE 700 1- Sela Ilan Virus Laboratory and the Otto Warburg Center for Biotechnology in Argiculture, The Hebrew University of Jerusalem, Faculty of Agriculture, 76100, Rehovot, Israel aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/1(1990-06-01), 27-39 0920-8569 4:1<27 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer