caa a22 4500 465795196 CHVBK 20180323112035.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00265631 doi (NATIONALLICENCE)springer-10.1007/BF00265631 Cloning and mapping of the potato virus Y genome and its in vitro expression [Elektronische Daten] [Miri Karchi, Ilan Sela, Orit Edelbaum, Assaf Ori, Benjamin Raccah, Arie Rosner, Yehuda Stram] Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein. Kluwer Academic Publishers, 1990 PVY nationallicence cloning nationallicence expression nationallicence viruses nationallicence Karchi Miri Virus Laboratory and the Otto Warburg Center for Biotechnology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel aut Sela Ilan Virus Laboratory and the Otto Warburg Center for Biotechnology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel aut Edelbaum Orit Virus Laboratory and the Otto Warburg Center for Biotechnology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel aut Ori Assaf Virus Laboratory and the Otto Warburg Center for Biotechnology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel aut Raccah Benjamin Department of Virology, Agricultural Research Organization The Volcani Center, Bet Dagan, Israel aut Rosner Arie Department of Virology, Agricultural Research Organization The Volcani Center, Bet Dagan, Israel aut Stram Yehuda Virus Laboratory and the Otto Warburg Center for Biotechnology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel aut Virus Genes Kluwer Academic Publishers 4/3(1990-09-01), 215-224 0920-8569 4:3<215 1990 4 11262 https://doi.org/10.1007/BF00265631 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00265631 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Karchi Miri Virus Laboratory and the Otto Warburg Center for Biotechnology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel aut NATIONALLICENCE 700 1- Sela Ilan Virus Laboratory and the Otto Warburg Center for Biotechnology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel aut NATIONALLICENCE 700 1- Edelbaum Orit Virus Laboratory and the Otto Warburg Center for Biotechnology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel aut NATIONALLICENCE 700 1- Ori Assaf Virus Laboratory and the Otto Warburg Center for Biotechnology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel aut NATIONALLICENCE 700 1- Raccah Benjamin Department of Virology, Agricultural Research Organization The Volcani Center, Bet Dagan, Israel aut NATIONALLICENCE 700 1- Rosner Arie Department of Virology, Agricultural Research Organization The Volcani Center, Bet Dagan, Israel aut NATIONALLICENCE 700 1- Stram Yehuda Virus Laboratory and the Otto Warburg Center for Biotechnology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/3(1990-09-01), 215-224 0920-8569 4:3<215 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer