caa a22 4500 465795234 CHVBK 20180323112035.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00570030 doi (NATIONALLICENCE)springer-10.1007/BF00570030 Investigation of the 5′ terminal structures of genomic and subgenomic RNAs of potato virus S [Elektronische Daten] [Gary Foster, Peter Mills] Hybrid arrest translation involving an antisense RNA, generated from a cloned cDNA close to the 5′ region of potato virus S genomic RNA, blocked the synthesis of genomic encoded products but had little effect on subgenomic RNA encoded products. Similarly, the synthesis of PVS genomic RNA-directed peptides was inhibited by the cap analogue m7G5′ ppp5′G, suggesting the presence of a cap structure at the 5′ terminus whilst subgenomic RNA encoded products remained unaffected, suggesting an uncapped structure. This was confirmed by artificially produced uncapped subgenomic RNAs translating as efficiently in in vitro translation systems as authentic viral subgenomic RNAs. Kluwer Academic Publishers, 1990 PVS nationallicence carlavirus nationallicence cloning nationallicence 5 cap structure nationallicence in vitro transcription nationallicence Foster Gary Department of Mycology and Plant Pathology, The Queen's University of Belfast, Belfast, Ireland aut Mills Peter Department of Mycology and Plant Pathology, The Queen's University of Belfast, Belfast, Ireland aut Virus Genes Kluwer Academic Publishers 4/4(1990-12-01), 359-366 0920-8569 4:4<359 1990 4 11262 https://doi.org/10.1007/BF00570030 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00570030 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Foster Gary Department of Mycology and Plant Pathology, The Queen's University of Belfast, Belfast, Ireland aut NATIONALLICENCE 700 1- Mills Peter Department of Mycology and Plant Pathology, The Queen's University of Belfast, Belfast, Ireland aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/4(1990-12-01), 359-366 0920-8569 4:4<359 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer