caa a22 4500 465795242 CHVBK 20180323112035.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00570024 doi (NATIONALLICENCE)springer-10.1007/BF00570024 Sequence analysis of the leader RNA of two porcine coronaviruses: Transmissible gastroenteritis virus and porcine respiratory coronavirus [Elektronische Daten] [Kevin Page, Paul Britton, Michael Boursnell] The leader RNA sequence was determined for two pig coronaviruses, tranmissible gastroenteritis virus (TGEV), and porcine respiratory coronavirus (PRCV). Primer extension, of a synthetic oligonucleotide complementary to the 5′ end of the nucleoprotein gene of TGEV was used to produce a single-stranded DNA copy of the leader RNA from the nucleoprotein mRNA species from TGEV and PRCV, the sequences of which were determined by Maxam and Gilbert cleavage. Northern blot analysis, using a synthetic oligonucleotide complementary to the leader RNA, showed that the leader RNA sequence was present on all of the subgenomic mRNA species. The porcine coronavirus leader RNA sequences were compared to each other and to published coronavirus leader RNA sequences. Sequence homologies and secondary structure similarities were identified that may play a role in the biological function of these RNA sequences. Kluwer Academic Publishers, 1990 transmissible gastroenteritis virus nationallicence porcine respiratory coronavirus nationallicence leader sequence nationallicence primer extension nationallicence Page Kevin Compton Laboratory, A.F.R.C., Institute for Animal Health, Compton, Newbury, Berkshire, UK aut Britton Paul Compton Laboratory, A.F.R.C., Institute for Animal Health, Compton, Newbury, Berkshire, UK aut Boursnell Michael Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire, UK aut Virus Genes Kluwer Academic Publishers 4/4(1990-12-01), 289-301 0920-8569 4:4<289 1990 4 11262 https://doi.org/10.1007/BF00570024 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00570024 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Page Kevin Compton Laboratory, A.F.R.C., Institute for Animal Health, Compton, Newbury, Berkshire, UK aut NATIONALLICENCE 700 1- Britton Paul Compton Laboratory, A.F.R.C., Institute for Animal Health, Compton, Newbury, Berkshire, UK aut NATIONALLICENCE 700 1- Boursnell Michael Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire, UK aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/4(1990-12-01), 289-301 0920-8569 4:4<289 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer