caa a22 4500 465795250 CHVBK 20180323112036.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00570025 doi (NATIONALLICENCE)springer-10.1007/BF00570025 Identification and localization of vpr gene product of human immunodeficiency virus type 1 [Elektronische Daten] [Akihiko Sato, Hisanaga Igarashi, Akio Adachi, Masanori Hayami] The entirevpr gene of human immunodeficiency virus type 1 (HIV-1) was cloned into procaryotic and eucaryotic expression vectors. Production of authentic protein encoded by the gene in bacterial and mammalian cells was monitored by Western blotting using guinea pig antisera raised against an N-terminal 14-oligopeptide of the predictedvpr protein. A specific 12-kD protein was clearly detected with these antisera, but not with preimmune sera, in both cell systems, and this binding was blocked by the oligopeptide. These antisera also recognized a protein of the same size in several human T-cell lines infected with HIV-1. Western blotting analysis of subcellular fractions prepared from the cells producing wildtypevpr protein strongly suggested that the protein was membrane associated. A region within thevpr required for the stable expression ofvpr product was also suggested by mutational analyses. Kluwer Academic Publishers, 1990 AIDS nationallicence HIV-1 nationallicence vpr nationallicence identification nationallicence localization nationallicence Western nationallicence Sato Akihiko Shionogi Institute for Medical Science, Settsu-shi, Osaka, Japan aut Igarashi Hisanaga Shionogi Institute for Medical Science, Settsu-shi, Osaka, Japan aut Adachi Akio Institute for Virus Research, Kyoto University, Kyoto, Japan aut Hayami Masanori Institute for Virus Research, Kyoto University, Kyoto, Japan aut Virus Genes Kluwer Academic Publishers 4/4(1990-12-01), 303-312 0920-8569 4:4<303 1990 4 11262 https://doi.org/10.1007/BF00570025 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00570025 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sato Akihiko Shionogi Institute for Medical Science, Settsu-shi, Osaka, Japan aut NATIONALLICENCE 700 1- Igarashi Hisanaga Shionogi Institute for Medical Science, Settsu-shi, Osaka, Japan aut NATIONALLICENCE 700 1- Adachi Akio Institute for Virus Research, Kyoto University, Kyoto, Japan aut NATIONALLICENCE 700 1- Hayami Masanori Institute for Virus Research, Kyoto University, Kyoto, Japan aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/4(1990-12-01), 303-312 0920-8569 4:4<303 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer