caa a22 4500 465795269 CHVBK 20180323112036.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00570026 doi (NATIONALLICENCE)springer-10.1007/BF00570026 Detection of human papillomavirus (HPV) type 16 and 52b in cervical cancer tissues by Southern blot hybridization and polymerase chain reaction (PCR) [Elektronische Daten] [Nobuo Yaegashi, Hajime Yajima, Kazuo Shikano, Ryo Konno, Shinji Sato, Kiichiro Noda, Akira Yajima] DNA samples from cancer tissues diagnosed histologically as squamous cell carcinoma of the uterine cervix were examined for the presence of human papillomavirus (HPV) genome DNA by Southern blot hybridization. Of 63 specimens, 32 were found to hybridize with HPV DNA probes; 23 specimens (35%) with HPV type 16 (HPV16), one (2%) with HPV type 18 (HPV18), four (7%) with HPV type 52b (HPV52b), and four others (7%) weakly with HPV52b. Specimens negative for HPV DNA with Southern blot hybridization were subjected to polymerase chain reaction (PCR) to determine the presence of HPV DNA under more sensitive conditions. After PCR using one set of primers specific for HPV16 and HPV52b, 7 out of 31 specimens were found to have HPV16 DNA. None was positive for HPV52b DNA. Our results indicate that HPV52b, as well as HPV16 and HPV18, is associated with squamous cell carcinoma of uterine cervix, and more sensitive determination of HPV infection can be made by amplification of the viral genome by PCR. Kluwer Academic Publishers, 1990 HPV16 nationallicence HPV52b nationallicence PCR nationallicence squamous cell carcinoma nationallicence uterine cervical cancer nationallicence Southern blot hybridization nationallicence Yaegashi Nobuo Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut Yajima Hajime Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut Shikano Kazuo Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut Konno Ryo Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut Sato Shinji Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut Noda Kiichiro Department of Obstetrics and Gynecology, Kinki University School of Medicine, Osaka, Japan aut Yajima Akira Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut Virus Genes Kluwer Academic Publishers 4/4(1990-12-01), 313-323 0920-8569 4:4<313 1990 4 11262 https://doi.org/10.1007/BF00570026 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00570026 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yaegashi Nobuo Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut NATIONALLICENCE 700 1- Yajima Hajime Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut NATIONALLICENCE 700 1- Shikano Kazuo Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut NATIONALLICENCE 700 1- Konno Ryo Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut NATIONALLICENCE 700 1- Sato Shinji Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut NATIONALLICENCE 700 1- Noda Kiichiro Department of Obstetrics and Gynecology, Kinki University School of Medicine, Osaka, Japan aut NATIONALLICENCE 700 1- Yajima Akira Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/4(1990-12-01), 313-323 0920-8569 4:4<313 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer