caa a22 4500 465795285 CHVBK 20180323112036.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00570031 doi (NATIONALLICENCE)springer-10.1007/BF00570031 Integrated defective replication units of hepatitis B virus [Elektronische Daten] [Peter Schranz, Hanswalter Zentgraf, Claus Schröder] Stable transformants of the human hepatoma cell line HepG2 were established that constitutively transcribe a DNA unit consisting of a stretch of hepatitis B virus DNA and of nonviral DNA conferring resistance to neomycin. Previously it had been shown that upon cotransfection of such transformants with replication-competent HBV DNA, transcripts of such units become reverse transcribed, demonstrating that DNA constructs can function as defective replication units. Transformed cell lines stably transcribing the defective replication units could be shown to use the transcriptional starts for the viral pregenome and for the large core antigen at a ratio of 9:1. Upon the induction of replicative processes in the transformed cells by transfection with replication-competent wild type (wt) DNA, defective pregenomes transcribed from the integrated state became included in the pool of replicating nucleic acids. Kluwer Academic Publishers, 1990 defective hepatitis B virus nationallicence integrants nationallicence replication nationallicence Schranz Peter Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, FRG aut Zentgraf Hanswalter Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, FRG aut Schröder Claus Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, FRG aut Virus Genes Kluwer Academic Publishers 4/4(1990-12-01), 367-374 0920-8569 4:4<367 1990 4 11262 https://doi.org/10.1007/BF00570031 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00570031 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schranz Peter Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, FRG aut NATIONALLICENCE 700 1- Zentgraf Hanswalter Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, FRG aut NATIONALLICENCE 700 1- Schröder Claus Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, FRG aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/4(1990-12-01), 367-374 0920-8569 4:4<367 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer