caa a22 4500 465795315 CHVBK 20180323112036.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00678404 doi (NATIONALLICENCE)springer-10.1007/BF00678404 RNA pseudoknots: Translational frameshifting and readthrough on viral RNAs [Elektronische Daten] [Edwin Ten Dam, Cornelius Pleij, Leendert Bosch] Ribosomal frameshifting on retroviral RNAs has been proposed to be mediated by slippage of two adjacent tRNAs into the — 1 direction at a specific heptanucleotide sequence. Here we report a computer-aided analysis of the structure around the established or putative frameshift sites in a number of retroviral, coronaviral, toroviral, and luteoviral RNAs and two dsRNA yeast viruses. In almost all cases a stable hairpin was predicted four to nine nucleotides downstream of the shifty heptanucleotide. More than half of the resulting hairpin loops give rise to potential pseudoknotting with sequences downstream of this hairpin. Especially in the case of the shifty heptanucleotides U UUA AAC and G GGA AAC, stable downstream pseudoknots are present. Indications were also found for the presence of pseudoknots downstream of amber stop condons at readthrough sites in some retroviral RNAs. Kluwer Academic Publishers, 1990 pseudoknots nationallicence ribosomal frameshifting nationallicence readthrough nationallicence RNA structure nationallicence Ten Dam Edwin Department of Biochemistry, University of Leiden, Leiden, The Netherlands aut Pleij Cornelius Department of Biochemistry, University of Leiden, Leiden, The Netherlands aut Bosch Leendert Department of Biochemistry, University of Leiden, Leiden, The Netherlands aut Virus Genes Kluwer Academic Publishers 4/2(1990-07-01), 121-136 0920-8569 4:2<121 1990 4 11262 https://doi.org/10.1007/BF00678404 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00678404 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ten Dam Edwin Department of Biochemistry, University of Leiden, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Pleij Cornelius Department of Biochemistry, University of Leiden, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Bosch Leendert Department of Biochemistry, University of Leiden, Leiden, The Netherlands aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/2(1990-07-01), 121-136 0920-8569 4:2<121 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer