caa a22 4500 465795331 CHVBK 20180323112036.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00678405 doi (NATIONALLICENCE)springer-10.1007/BF00678405 Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis virus. I. Nucleotide sequence of the nucleoprotein gene [Elektronische Daten] [Katsuhiro Komase, Tatsuhiko Kasaoka, Yasuhiro Yoshikawa, Takeshi Sato, Kazuya Yamanouchi] The complete nucleotide sequence of cloned cDNAs corresponding to the fulllength mRNA encoding the NP protein of the Yamagata-1 strain of subacute sclerosing panencephalitis (SSPE) virus was determined. The gene is composed of 1683 nucleotides and contains a single large open reading frame, which is capable of encoding 525 amino acids with a molecular weight of 58,399. Comparison of the nucleotide and predicted amino acid sequences with those of the Edmonston strain of measles virus (MV) showed that the gene and the protein were highly conserved. However, the antigenic sites on the NP protein of the Yamagata-1 strain were found to be changed by an eiptope analysis using monoclonal antibodies against the NP protein of MV. Only 1 of 4 monoclonal antibodies reacted with the NP protein of SSPE virus, and the other three antibodies did not. Almost identical changes in nucleotides and amino acids were found to occur in the NP gene of the Yamagata-1 strain when compared with the IP-3-Ca strain of another SSPE virus. In addition, the deduced secondary structure of the NP protein of the IP-3-Ca strain was similar to that of the Yamagata-1 strain, but differed from the MV. These results suggest that the NP proteins of SSPE viruses have a common property that is different from MV. Kluwer Academic Publishers, 1990 SSPE virus nationallicence measles virus nationallicence nucleoprotein gene nationallicence sequence analysis nationallicence Komase Katsuhiro Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut Kasaoka Tatsuhiko Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut Yoshikawa Yasuhiro Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut Sato Takeshi Department of Measles Virus, National Institute of Health of Japan, Tokyo, Japan aut Yamanouchi Kazuya Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut Virus Genes Kluwer Academic Publishers 4/2(1990-07-01), 137-149 0920-8569 4:2<137 1990 4 11262 https://doi.org/10.1007/BF00678405 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00678405 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Komase Katsuhiro Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Kasaoka Tatsuhiko Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Yoshikawa Yasuhiro Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Sato Takeshi Department of Measles Virus, National Institute of Health of Japan, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamanouchi Kazuya Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/2(1990-07-01), 137-149 0920-8569 4:2<137 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 465795331 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer