caa a22 4500 465795366 CHVBK 20180323112036.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00678408 doi (NATIONALLICENCE)springer-10.1007/BF00678408 Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis virus. IV. Nucleotide sequence of the fusion gene [Elektronische Daten] [Katsuhiro Komase, Takeshi Haga, Yasuhiro Yoshikawa, Takeshi Sato, Kazuya Yamanouchi] The full-length cDNA corresponding to the mRNA of the fusion (F) protein of the Yamagata-1 strain of subacute sclerosing panencephalitis (SSPE) virus was cloned, and its complete nucleotide sequence was determined. The F gene was composed of 2369 nucleotides and contained a single large coding region, which is located between two noncoding regions. The 5′-terminal noncoding region consisted of 584 nucleotides comprising 44.9% cytosine, and had several inverted repetitious sequences. The 3′-terminal noncoding region had a relatively low homology of 91.7% with the MV. The coding region was expanded for nucleotides 585-2189, which encoded 534 amino acids with a molecular weight of 57,963. The homology of the amino acid sequence of the F protein between the MV and SSPE virus was 96.27%, and the positions of cysteine and proline were almost identical in the two viruses. The functional domains of SSPE-virus F protein closely resembled those of MV F protein, including the cleavage site, a signal sequence, the fusion-related stretch, the transmembrane region, and four potential glycosylation sites. Four antigenic epitopes on the MV F protein were also conserved on the SSPE-virus F protein. However, deletion of one nucleotide (position 2155) of the SSPE virus was found when compared with the MV, and shifted the coding frame, causing the substitutions of 27 C-terminal amino acids of the MV F protein with 11 different residues. The variations of the C-terminal region of the F protein were observed with two other SSPE viruses, suggesting that this may be a common property of SSPE virus that differs from MV. Kluwer Academic Publishers, 1990 SSPE virus nationallicence measles virus nationallicence fusion gene nationallicence sequence analysis nationallicence Komase Katsuhiro Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut Haga Takeshi Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut Yoshikawa Yasuhiro Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut Sato Takeshi Department of Measles Virus, National Institute of Health of Japan, Tokyo, Japan aut Yamanouchi Kazuya Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut Virus Genes Kluwer Academic Publishers 4/2(1990-07-01), 173-181 0920-8569 4:2<173 1990 4 11262 https://doi.org/10.1007/BF00678408 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00678408 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Komase Katsuhiro Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Haga Takeshi Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Yoshikawa Yasuhiro Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Sato Takeshi Department of Measles Virus, National Institute of Health of Japan, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamanouchi Kazuya Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/2(1990-07-01), 173-181 0920-8569 4:2<173 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 465795331 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer