caa a22 4500 465795382 CHVBK 20180323112036.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00393181 doi (NATIONALLICENCE)springer-10.1007/BF00393181 Transient expression assay for qualitative assessment of gene expression by fowlpox virus [Elektronische Daten] [Shree Dhawale, Christopher Beisel, Keyvan Nazerian] A transient expression assay for fowlpox virus (FPV) was developed to assess the feasibility of using heterologous promoters in FPV and to qualitatively determine relative promoter strength. A transient expression system for FPV has not been reported, and various methods used for transient expression in vaccinia-virus-infected cells produced negative results when used with FPV. Here a successful method for transient expression of E. coli beta-galactosidase in FPV-infected chick embryo fibroblasts is reported. This transient expression assay has been developed to qualitatively assess promoter recognition and gene expression by FPV. It should also prove useful in the identification of promoters from the FPV genomic library and in testing the accuracy of chimeric promoter-gene constructs. Klumer Academic Publishers, 1990 fowlpox virus nationallicence transient expression nationallicence viral vectors nationallicence avian expression vector nationallicence Dhawale Shree Agricultural Research Service, Regional Poultry Research Laboratory, USDA, East Lansing, MI, USA aut Beisel Christopher Agricultural Research Service, Regional Poultry Research Laboratory, USDA, East Lansing, MI, USA aut Nazerian Keyvan Agricultural Research Service, Regional Poultry Research Laboratory, USDA, East Lansing, MI, USA aut Virus Genes Kluwer Academic Publishers 3/3(1990-02-01), 213-220 0920-8569 3:3<213 1990 3 11262 https://doi.org/10.1007/BF00393181 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00393181 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dhawale Shree Agricultural Research Service, Regional Poultry Research Laboratory, USDA, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Beisel Christopher Agricultural Research Service, Regional Poultry Research Laboratory, USDA, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Nazerian Keyvan Agricultural Research Service, Regional Poultry Research Laboratory, USDA, East Lansing, MI, USA aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 3/3(1990-02-01), 213-220 0920-8569 3:3<213 1990 3 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer