caa a22 4500 465795455 CHVBK 20180323112036.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00393180 doi (NATIONALLICENCE)springer-10.1007/BF00393180 Balázs Ervin Biotechnology Department, Plant Protection Institute, Budapest, Hungary aut Disease symptoms in transgenic tobacco induced by integrated gene VI of cauliflower mosaic virus [Elektronische Daten] [Ervin Balázs] A chimeric vector (pKR 612B1) containing the neomycin phosphotransferase (APH) gene from the Tn5 transposon under the control of the gene VI promoter of cauliflower mosaic virus (CaMV) and the cloned gene VI region (SalI-BstEII) of the same virus were used to cotransform tobacco protoplasts. Using the polyethylene glycol transformation procedure, a large number of protoplasts were transformed and proved to be resistant to kanamycin (Km). Whole Km-resistant plants were regenerated and shown to contain the integrated foreign genes. DNA from transformed clones was analyzed by Southern blot hybridization, showing the presence of the Tn5-derived gene and the viral gene. Transgenic plants containing the viral gene show mild mosaic patterns and fasciation. The expression of the gene VI product was detected by immunoblots. Klumer Academic Publishers, 1990 transgenic tobacco nationallicence cauliflower mosaic virus nationallicence viral gene functions nationallicence Virus Genes Kluwer Academic Publishers 3/3(1990-02-01), 205-211 0920-8569 3:3<205 1990 3 11262 https://doi.org/10.1007/BF00393180 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00393180 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Balázs Ervin Biotechnology Department, Plant Protection Institute, Budapest, Hungary aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 3/3(1990-02-01), 205-211 0920-8569 3:3<205 1990 3 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer