caa a22 4500 46579548X CHVBK 20180323112036.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00569040 doi (NATIONALLICENCE)springer-10.1007/BF00569040 Neutralization of purified herpes simplex virus DNA polymerase by two antipeptide sera [Elektronische Daten] [James Matthews, John Stevens, Brian Terry, Christopher Cianci, Mary Haffey] Synthetic peptides corresponding to amino acid sequences present in the herpes simplex virus type-1 (HSV-1) DNA polymerase (pol) were used to raise polyclonal rabbit antisera. The three peptides described in detail in this report were among seven sequences chosen for initial studies designed to generate reagents capable of recognizing discrete regions of the HSV-1 pol protein from the amino to carboxy termini. Two of the peptides, designated P6 and P7, representing amino acid residues 1100-1108 and 1216-1224 of the deduced HSV-1 (strain KOS) DNA pol sequence (1235 residues) produced antisera that could not only recognize the native HSV-1 pol enzyme but also could specifically neutralize purified HSV-1 pol activity in a dose-dependent manner. An additional peptide, designated P3, representing residues 548-557, produced an antiserum that was unable to recognize the native protein but could react with HSV-1 pol in a denatured form by immunoblot assay. Kluwer Academic Publishers, 1990 HSV-1 nationallicence HSV-1 pol protein nationallicence HSV DNA polymerase nationallicence antipeptide sera nationallicence Matthews James Department of Virology, The Squibb Institute for Medical Research, Princeton, NJ, USA aut Stevens John Department of Virology, The Squibb Institute for Medical Research, Princeton, NJ, USA aut Terry Brian Department of Virology, The Squibb Institute for Medical Research, Princeton, NJ, USA aut Cianci Christopher Department of Virology, The Squibb Institute for Medical Research, Princeton, NJ, USA aut Haffey Mary Department of Virology, The Squibb Institute for Medical Research, Princeton, NJ, USA aut Virus Genes Kluwer Academic Publishers 3/4(1990-04-01), 343-354 0920-8569 3:4<343 1990 3 11262 https://doi.org/10.1007/BF00569040 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00569040 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matthews James Department of Virology, The Squibb Institute for Medical Research, Princeton, NJ, USA aut NATIONALLICENCE 700 1- Stevens John Department of Virology, The Squibb Institute for Medical Research, Princeton, NJ, USA aut NATIONALLICENCE 700 1- Terry Brian Department of Virology, The Squibb Institute for Medical Research, Princeton, NJ, USA aut NATIONALLICENCE 700 1- Cianci Christopher Department of Virology, The Squibb Institute for Medical Research, Princeton, NJ, USA aut NATIONALLICENCE 700 1- Haffey Mary Department of Virology, The Squibb Institute for Medical Research, Princeton, NJ, USA aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 3/4(1990-04-01), 343-354 0920-8569 3:4<343 1990 3 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer