caa a22 4500 465795498 CHVBK 20180323112036.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00569041 doi (NATIONALLICENCE)springer-10.1007/BF00569041 Anchoring a vaccinia virus promoter in the nucleus prevents its trans -activation by viral infection [Elektronische Daten] [Jeffery Miner, Azaibi Tamin, Dennis Hruby] The vaccinia virus 7.5 kDa constitutive promoter, when fused to a reporter gene and recombined into the genome of L cells, is not activatable upon subsequent infection with vaccinia virus. However, the same promoter is actively transcribed during transient cytoplasmic transfection procedures or within the context of the viral genome. This suggests that the intact vaccinia transcriptional machinery either does not enter the nucleus or, if it does, is unable to interact with cellular chromatin. Kluwer Academic Publishers, 1990 transient expression nationallicence CAT gene nationallicence transcription nationallicence Miner Jeffery Department of Biochemistry, University of California, San Francisco, San Francisco, CA, USA aut Tamin Azaibi Poxvirus Laboratories, Centers for Disease Control, 1600 Clifton Rd. N.E., Mail Stop G-18, Atlanta, GA, USA aut Hruby Dennis Center for Gene Research, Department of Microbiology, Oregon State University, Corvallis, OR, USA aut Virus Genes Kluwer Academic Publishers 3/4(1990-04-01), 355-359 0920-8569 3:4<355 1990 3 11262 https://doi.org/10.1007/BF00569041 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00569041 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miner Jeffery Department of Biochemistry, University of California, San Francisco, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Tamin Azaibi Poxvirus Laboratories, Centers for Disease Control, 1600 Clifton Rd. N.E., Mail Stop G-18, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Hruby Dennis Center for Gene Research, Department of Microbiology, Oregon State University, Corvallis, OR, USA aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 3/4(1990-04-01), 355-359 0920-8569 3:4<355 1990 3 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer