caa a22 4500 46579551X CHVBK 20180323112036.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00569038 doi (NATIONALLICENCE)springer-10.1007/BF00569038 Marek's disease virus gene clones encoding virus-specific phosphorylated polypeptides and serological characterization of fusion proteins [Elektronische Daten] [Zhizhong Cui, Ding Yan, Lucy Lee] Marek's disease virus (MDV) gene clones, RA2 and GA8, constructed inE. coli bacteriophage lambda-gt11 (gt11) were identified by a monoclonal antibody (MAb), H19.47, against a putative transformation-related viral antigen consisting of a complex of three phosphorylated polypeptides, pp41, pp38, and pp24. Both recombinants have a MDV-DNA insert of about 0.5 kb and are mapped to the region ofBamHI-H orEcoRI-X fragments of the MDV genome by Southern blot hybridization. Immunoblot and immunoprecipitation with H19.47 identified a recombinant beta-galactosidase-MDV 140-kD fusion protein for RA2 and a 127-kD fusion protein for GA8. Immunoprecipitation of35S-methionine-labeled, MDV-infected chicken embryo fibroblasts (CEF) with antisera against RA2 and GA8 fusion proteins recognized five polypeptides, of which three (p41, p38, and p24) are specified by H19.47 and the remaining two, p135 and p20, have not been previously identified. Immunoprecipitation of32P-phosphate-labeled or3H-glucosamine-labeled, GA-MDV-infected CEF with the antiserum against RA2 fusion protein identified a phosphorylated polypeptide of 38 kD and two glycoproteins of 60 and 49 kD, respectively. The antisera against recombinant fusion proteins thus revealed the existence of epitopes common to the phosphorylated polypeptides and other MDV-specific polypeptides. Sera from chickens or mice hyperimmunized with the purified fusion proteins reacted with serotype 1, MDV-infected CEF in the fluorescent antibody (FA) test to significant titers. These immune sera did not react with either serotype II or III, indicating the serotype specificity of the phosphorylated polypeptides. Kluwer Academic Publishers, 1990 lambda gt11 nationallicence MDV gene nationallicence phosphorylated proteins nationallicence Cui Zhizhong Department of Animal Science, Michigan State University, East Lansing, MI, USA aut Yan Ding Department of Animal Science, Michigan State University, East Lansing, MI, USA aut Lee Lucy Agricultural Research Service, Regional Poultry Research Laboratory, USDA, East Lansing, MI, USA aut Virus Genes Kluwer Academic Publishers 3/4(1990-04-01), 309-322 0920-8569 3:4<309 1990 3 11262 https://doi.org/10.1007/BF00569038 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00569038 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cui Zhizhong Department of Animal Science, Michigan State University, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Yan Ding Department of Animal Science, Michigan State University, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Lee Lucy Agricultural Research Service, Regional Poultry Research Laboratory, USDA, East Lansing, MI, USA aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 3/4(1990-04-01), 309-322 0920-8569 3:4<309 1990 3 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer