caa a22 4500 465795536 CHVBK 20180323112036.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00569037 doi (NATIONALLICENCE)springer-10.1007/BF00569037 Characterization of the gene encoding herpesvirus of turkeys gp57-65: Comparison to Marek's disease virus gp57-65 and herpes simplex virus glycoprotein C [Elektronische Daten] [Paul Coussens, Melinda Wilson, Heidi Camp, Holger Roehl, Robert Isfort, Leland Velicer] A gene encoding herpesvirus of turkeys (HVT) strain FC 126 gp57-65 has been mapped to the viral genome and sequenced. The HVT (FC 126) gp57-65 gene maps toBamHI fragments K1 and M, colinear with the gene from Marek's disease virus (MDV) strain GA. HVT gp57-65 gene sequences were compared to the MDV strain GA gp57-65 gene that we sequenced previously. Overall, the two sequences are 66% identical, with greater similarity in the 3′ proximal two thirds of the genes. HVT gp57-65 gene sequences have a slightly higher overall guanosine plus cytosine (G+C) content than MDV gp57-65 gene sequences (46% vs. 41%, respectively). A single, long open reading frame capable of encoding 523 amino acids was identified within the HVT gp57-65 gene region. The predicted precursor polypeptide derived from this open reading frame would have a calculated molecular weight of 58,587. The predicted HVT gp57-65 amino acid sequences contain six potential N-linked glycosylation sites (asn-x-ser/thr). Five of these six potential N-linked glycosylation sites are conserved between the HVT and MDV predicted amino acid sequences. Hydropathic analysis of the predicted HVT gp57-65 amino acid sequences indicate the presence of an amino-terminal hydrophobic sequence, which may function as a signal peptide, and a hydrophobic carboxyl terminal sequence, which may function as a membrane anchor sequence. Overall, MDV gp57-65 and HVT gp57-65 precursor polypeptide sequences are 73% homologous and share many potential antigenic epitopes. Predicted MDV and HVT gp57-65 protein sequences are similar to those of herpes simplex virus glycoprotein C (gC) and gC-like proteins from other herpesviruses. Similarities are scattered throughout the molecule, with a primary concentration near the carboxyl half of the molecule. One stretch of 60 amino acids (HVT amino acids 378-437 and MDV amino acids 350-410) are relatively well conserved among gC-like proteins from six herpesviruses. The possible implications of these homologies and the potential roles of gC-like proteins in virus infection, growth, and replication are discussed. Kluwer Academic Publishers, 1990 Marek's disease virus nationallicence herpesvirus of turkeys nationallicence glycoprotein nationallicence gp57-65 nationallicence gene sequence nationallicence Coussens Paul Department of Animal Science, Michigan State University, 48824, East Lansing, MI, USA aut Wilson Melinda Department of Animal Science, Michigan State University, 48824, East Lansing, MI, USA aut Camp Heidi Department of Animal Science, Michigan State University, 48824, East Lansing, MI, USA aut Roehl Holger Department of Microbiology and Public Health, Michigan State University, 48824, East Lansing, MI, USA aut Isfort Robert Department of Microbiology and Public Health, Michigan State University, 48824, East Lansing, MI, USA aut Velicer Leland Department of Microbiology and Public Health, Michigan State University, 48824, East Lansing, MI, USA aut Virus Genes Kluwer Academic Publishers 3/4(1990-04-01), 291-307 0920-8569 3:4<291 1990 3 11262 https://doi.org/10.1007/BF00569037 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00569037 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Coussens Paul Department of Animal Science, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Wilson Melinda Department of Animal Science, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Camp Heidi Department of Animal Science, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Roehl Holger Department of Microbiology and Public Health, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Isfort Robert Department of Microbiology and Public Health, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Velicer Leland Department of Microbiology and Public Health, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 3/4(1990-04-01), 291-307 0920-8569 3:4<291 1990 3 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer