caa a22 4500 465795544 CHVBK 20180323112036.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00569043 doi (NATIONALLICENCE)springer-10.1007/BF00569043 Herpes simplex virus-encoded ribonucleotide reductase: Evidence for the dissociation/ reassociation of the holoenzyme [Elektronische Daten] [Allan Darling, Elizabeth McKay, Rolf Ingemarson, Brian Booth] 35S-labeled cells infected with herpes simplex virus type 1 (HSV-1), temperature-sensitive (ts) mutantts 1222 were used as a source of the large subunit of the viral ribonucleotide reductase (RR) to investigate the binding of the large (RR1) and small (RR2) subunits in the active enzyme. Mixing35S-labeled RR1 fromts 1222 with unlabeled RR1/RR2 complex from wild type (wt) infected cells resulted in the formation of a complex between35S-labeled RR1 and unlabeled RR2, indicating that the complex between the RR1 and RR2 subunits is dynamic and subunit dissociation/reassociation occurs during enzyme function. Similar results were obtained when unlabeled HSV-2 RR was substituted for HSV-1 RR, demonstrating that the holoenzyme can be formed from the large subunit of HSV-1 RR and the small subunit of HSV-2. Kluwer Academic Publishers, 1990 HSV nationallicence ribonucleotide reductase nationallicence subunit dissociation nationallicence reassociation nationallicence Darling Allan M.R.C. Virology Unit, Institute of Virology, Glasgow, Scotland aut McKay Elizabeth M.R.C. Virology Unit, Institute of Virology, Glasgow, Scotland aut Ingemarson Rolf Department of Physiological Chemistry, University of Umea, Umea, Sweden aut Booth Brian Glaxo Group Research Ltd., Greenford, Middlesex, England aut Virus Genes Kluwer Academic Publishers 3/4(1990-04-01), 367-372 0920-8569 3:4<367 1990 3 11262 https://doi.org/10.1007/BF00569043 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00569043 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Darling Allan M.R.C. Virology Unit, Institute of Virology, Glasgow, Scotland aut NATIONALLICENCE 700 1- McKay Elizabeth M.R.C. Virology Unit, Institute of Virology, Glasgow, Scotland aut NATIONALLICENCE 700 1- Ingemarson Rolf Department of Physiological Chemistry, University of Umea, Umea, Sweden aut NATIONALLICENCE 700 1- Booth Brian Glaxo Group Research Ltd., Greenford, Middlesex, England aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 3/4(1990-04-01), 367-372 0920-8569 3:4<367 1990 3 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer