caa a22 4500 465795714 CHVBK 20180323112037.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02539689 doi (NATIONALLICENCE)springer-10.1007/BF02539689 Isolation and physicochemical characterization of the half-unit membranes of oilseed lipid bodies [Elektronische Daten] [T. Jacks, T. Hensarling, J. Neucere, L. Yatsu, R. Barker] The lipid-free residue of lipid body membranes was isolated from quiescent peanuts and was physicochemically characterized. The preponderant component of the residue was proteinaceous and consisted of at least two polypeptides according to ultracentrifugation, gel filtration, gel electrophoresis and HPLC. The molecular weight of the principal polypeptide was between 10,000 and 16,000 daltons. Only one antigen, immunochemically unique with respect to other peanut components, was detected. Spectral analyses indicated the presence of a protoheme and revealed conformational modes of β-sheet and unordered structure but no α-helix. The amino acid composition was similar to that of an integral membrane polypeptide rather than to those of peripheral membranes or other plant polypeptides. The hydrophobicity, conformation and quantitative content of polypeptides were suitable for the existence of a monolayer at the lipid body-cytoplasm interface. The results indicated that lipid body coatings physicochemically resemble membranes of intracellular organelles and supported the morphological concept that the coatings are half-unit biological membranes. Reutilization of lipid body membranes appeared possible after lipid depletion during seed germination. AOCS Press, 1990 Jacks T. Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA aut Hensarling T. Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA aut Neucere J. Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA aut Yatsu L. Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA aut Barker R. American Fiber Manufacturers Association, 20036, Washington, DC aut Journal of the American Oil Chemists' Society Springer-Verlag 67/6(1990-06-01), 353-361 0003-021X 67:6<353 1990 67 11746 https://doi.org/10.1007/BF02539689 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02539689 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jacks T. Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA aut NATIONALLICENCE 700 1- Hensarling T. Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA aut NATIONALLICENCE 700 1- Neucere J. Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA aut NATIONALLICENCE 700 1- Yatsu L. Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA aut NATIONALLICENCE 700 1- Barker R. American Fiber Manufacturers Association, 20036, Washington, DC aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/6(1990-06-01), 353-361 0003-021X 67:6<353 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer