caa a22 4500 465795919 CHVBK 20180323112037.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF02540767 doi (NATIONALLICENCE)springer-10.1007/BF02540767 Enzymatic fractionation of fatty acids: Enrichment of γ-linolenic acid and docosahexaenoic acid by selective esterification catalyzed by lipases [Elektronische Daten] [Matthew Hills, Irmgard Kiewitt, Kumar Mukherjee] Immobilized lipase preparations from seedlings of rape (Brassica napus L.) andMucor miehei (lipozyme) used as biocatalysts in esterification and hydrolysis reactions discriminate strongly against γ-linolenic and docosahexaenoic acids/acyl moieties. Utilizing this property, γ-linolenic acid contained in fatty acids of evening primrose oil has been enriched seven to nine-fold, from 9.5 to almost 85% by selective esterification of the other fatty acids with butanol. Similarly, docosahexaenoic acid of cod liver oil has been enriched four to five-fold, from 9.4 to 45% by selective esterification of fatty acids (other than docosahexaenoic acid) with butanol. As long as the reaction is stopped before reaching equilibrium, very little of either γ-linolenic acid or docosahexaenoic acid are converted to butyl esters, which results in high yields of these acids in the unesterified fatty acid fraction. AOCS Press, 1990 Cod liver oil nationallicence docosahexaenoic acid nationallicence enzymatic fractionation of fatty acids nationallicence evening primrose oil nationallicence γ-linolenic acid nationallicence lipase-catalyzed esterification nationallicence Mucor miehei lipase nationallicence rape lipase nationallicence Hills Matthew Federal Center for Lipid Research, Institute for Biochemistry and Technology, H.P. Kaufmann-Institute, Piusallee 68, D-4400, Münster, Feaoral Republic of Germany aut Kiewitt Irmgard Federal Center for Lipid Research, Institute for Biochemistry and Technology, H.P. Kaufmann-Institute, Piusallee 68, D-4400, Münster, Feaoral Republic of Germany aut Mukherjee Kumar Federal Center for Lipid Research, Institute for Biochemistry and Technology, H.P. Kaufmann-Institute, Piusallee 68, D-4400, Münster, Feaoral Republic of Germany aut Journal of the American Oil Chemists' Society Springer-Verlag 67/9(1990-09-01), 561-564 0003-021X 67:9<561 1990 67 11746 https://doi.org/10.1007/BF02540767 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02540767 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hills Matthew Federal Center for Lipid Research, Institute for Biochemistry and Technology, H.P. Kaufmann-Institute, Piusallee 68, D-4400, Münster, Feaoral Republic of Germany aut NATIONALLICENCE 700 1- Kiewitt Irmgard Federal Center for Lipid Research, Institute for Biochemistry and Technology, H.P. Kaufmann-Institute, Piusallee 68, D-4400, Münster, Feaoral Republic of Germany aut NATIONALLICENCE 700 1- Mukherjee Kumar Federal Center for Lipid Research, Institute for Biochemistry and Technology, H.P. Kaufmann-Institute, Piusallee 68, D-4400, Münster, Feaoral Republic of Germany aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/9(1990-09-01), 561-564 0003-021X 67:9<561 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer