caa a22 4500 465796338 CHVBK 20180323112038.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02541846 doi (NATIONALLICENCE)springer-10.1007/BF02541846 Production of eicosapentaenoic acid by a bacterium isolated from mackerel intestines [Elektronische Daten] [Masamichi Akimoto, Tetsuya Ishii, Koji Yamagaki, Kazuhisa Ohtaguchi, Kozo Koide, Kazunaga Yazawa] Optimization of culture conditions for the growth rate, 5,8,11,14,17-cis-Eicosapentaenoic acid (EPA) content and EPA productivity of a bacterium isolated from Pacific mackerel intestines was investigated by use of a culture medium containing 1.00 wt% peptone and 0.50 wt% yeast extract in an artificial sea water (ASW). Cultivation temperature affected the growth rate and cellular EPA content of the bacterium. The cellular EPA content at 8°C was as great as 16.8 mg/g of dry cells, which was more than two times greater than that at 25°C (7.3 mg/g of dry cells), although the growth rate showed a maximum at 25°C. Both the yield of bacterial cells and the cellular EPA content at 25°C reached maximum values when the pH of the culture medium was nearly 7.0 and when the concentration of ASW was 100% (v/v). Under optimum culture conditions [25°C pH 7.0 and 100% (v/v) ASW], the amount of EPA accumulated in the cellular lipids reached 45.6 mg/L of culture broth after 8 hr. American Oil Chemists' Society (AOCS), 1990 Alteromonas putrefaciens nationallicence eicosapentaenoic acid nationallicence mackerel nationallicence marine bacterium nationallicence Akimoto Masamichi Department of Chemical Engineering, Tokyo Institute of Technology, Ookayama, Meguro-ku, 152, Tokyo, Japan aut Ishii Tetsuya Department of Chemical Engineering, Tokyo Institute of Technology, Ookayama, Meguro-ku, 152, Tokyo, Japan aut Yamagaki Koji Department of Chemical Engineering, Tokyo Institute of Technology, Ookayama, Meguro-ku, 152, Tokyo, Japan aut Ohtaguchi Kazuhisa Department of Chemical Engineering, Tokyo Institute of Technology, Ookayama, Meguro-ku, 152, Tokyo, Japan aut Koide Kozo Department of Chemical Engineering, Tokyo Institute of Technology, Ookayama, Meguro-ku, 152, Tokyo, Japan aut Yazawa Kazunaga Sagami Chemical Research Center, Nishi-ohnuma, 229, Sagamihara, Kanagawa, Japan aut Journal of the American Oil Chemists' Society Springer-Verlag 67/12(1990-12-01), 911-915 0003-021X 67:12<911 1990 67 11746 https://doi.org/10.1007/BF02541846 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02541846 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Akimoto Masamichi Department of Chemical Engineering, Tokyo Institute of Technology, Ookayama, Meguro-ku, 152, Tokyo, Japan aut NATIONALLICENCE 700 1- Ishii Tetsuya Department of Chemical Engineering, Tokyo Institute of Technology, Ookayama, Meguro-ku, 152, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamagaki Koji Department of Chemical Engineering, Tokyo Institute of Technology, Ookayama, Meguro-ku, 152, Tokyo, Japan aut NATIONALLICENCE 700 1- Ohtaguchi Kazuhisa Department of Chemical Engineering, Tokyo Institute of Technology, Ookayama, Meguro-ku, 152, Tokyo, Japan aut NATIONALLICENCE 700 1- Koide Kozo Department of Chemical Engineering, Tokyo Institute of Technology, Ookayama, Meguro-ku, 152, Tokyo, Japan aut NATIONALLICENCE 700 1- Yazawa Kazunaga Sagami Chemical Research Center, Nishi-ohnuma, 229, Sagamihara, Kanagawa, Japan aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/12(1990-12-01), 911-915 0003-021X 67:12<911 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer