caa a22 4500 465796435 CHVBK 20180323112039.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF02539622 doi (NATIONALLICENCE)springer-10.1007/BF02539622 Determination of free and total gossypol by high performance liquid chromatography [Elektronische Daten] [R. Hron Sr., M. Kuk, G. Abraham] Existing HPLC methods determine only pure gossypol whereas the official AOCS method determines both gossypol and other physiologically active gossypol-like compounds that react with 3-amino-1-propanol and aniline. The feed industry uses the official AOCS method, which is complex and produces results that do not correlate well among laboratories. HPLC methods were developed, using 3-amino-1-propanol as a complexing agent, for the quantitative determination of free and total gossypol in cottonseed meal, oil, and ethanolic miscella. These methods are simple, sensitive, and provide reproducible results. In addition the use of toxic aniline is eliminated. American Oil Chemists' Society, 1990 Hron Sr. R. Southern Regional Research Center, Agricultural Research Service, USDA, P.O. Box 19687, 70179, New Orleans, LA aut Kuk M. Southern Regional Research Center, Agricultural Research Service, USDA, P.O. Box 19687, 70179, New Orleans, LA aut Abraham G. Southern Regional Research Center, Agricultural Research Service, USDA, P.O. Box 19687, 70179, New Orleans, LA aut Journal of the American Oil Chemists' Society Springer-Verlag 67/3(1990-03-01), 182-187 0003-021X 67:3<182 1990 67 11746 https://doi.org/10.1007/BF02539622 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02539622 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hron Sr R. Southern Regional Research Center, Agricultural Research Service, USDA, P.O. Box 19687, 70179, New Orleans, LA aut NATIONALLICENCE 700 1- Kuk M. Southern Regional Research Center, Agricultural Research Service, USDA, P.O. Box 19687, 70179, New Orleans, LA aut NATIONALLICENCE 700 1- Abraham G. Southern Regional Research Center, Agricultural Research Service, USDA, P.O. Box 19687, 70179, New Orleans, LA aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/3(1990-03-01), 182-187 0003-021X 67:3<182 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer