caa a22 4500 465796591 CHVBK 20180323112039.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF02540413 doi (NATIONALLICENCE)springer-10.1007/BF02540413 Determination and occurrence of oxofatty acids in fats and oils [Elektronische Daten] [Daniel Schwartz, Aly Rady] A relatively simple method is detailed for the routine isolation and estimation of oxofatty acids (OFA) in lipids. The lipid in cyclohexane is transmethylated in a two-phase, 3.5 min procedure, and the carbonyls in the methyl ester fraction are derivatized with 2,4-dinitrophenylhydrazine (DNPH) in the presence of monochloroacetic acid (MCA). The derivatives are fractionated on alumina, and the OFA fraction is obtained and evaluated spectrophotometrically. A large variety of animal, plant, and marine lipids contained OFA ranging from <1 to >50 μmoles/g. Data also show that (a) OFA are formed in naturally oxidizing fats and oils, and (b) strongly acidic conditions can cause elaboration of OFA in hydroperoxidized fats and oils. AOCS Press, 1990 Alumina fractionation nationallicence 2,4-dinitrophenylhydrazones nationallicence fat oxidation nationallicence monochloroacetic acid nationallicence Schwartz Daniel Eastern Regional Research Center, ARS/USDA, 600 E. Mermald Lane, 19118, Philadelphia, PA aut Rady Aly Eastern Regional Research Center, ARS/USDA, 600 E. Mermald Lane, 19118, Philadelphia, PA aut Journal of the American Oil Chemists' Society Springer-Verlag 67/10(1990-10-01), 635-641 0003-021X 67:10<635 1990 67 11746 https://doi.org/10.1007/BF02540413 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02540413 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schwartz Daniel Eastern Regional Research Center, ARS/USDA, 600 E. Mermald Lane, 19118, Philadelphia, PA aut NATIONALLICENCE 700 1- Rady Aly Eastern Regional Research Center, ARS/USDA, 600 E. Mermald Lane, 19118, Philadelphia, PA aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/10(1990-10-01), 635-641 0003-021X 67:10<635 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer