caa a22 4500 46579680X CHVBK 20180323112040.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF02540754 doi (NATIONALLICENCE)springer-10.1007/BF02540754 Preparative chromatographic isolation of hydroxy acids from Lesquerella fendleri and L. gordonii seed oils [Elektronische Daten] [K. Carlson, A. Chaudhry, R. Peterson, M. Bagby] To conduct product development research onLesquerella seed oils, we explored methods to obtain >100 g quantities of lesquerolic (14-hydroxy-cis-11-eicosenoic) acid. Preliminary experiments with open-column silica gel chromatography showed thatL. fendleri oil could be separated into 3 triglyceride (TG) fractions. The first (10%) contained nonhydroxy 16-(13%) and 18-carbon acids (65% 18∶1,2,3). The second fraction (15%) contained monolesquerolins (39% lesquerolic acid). The major TG fraction (73%) was mainly dilesquerolins (66% lesquerolic acid) showing that a hydroxy acid-enriched TG oil was obtainable by this procedure. Silica gel chromatography easily separatedL. fendleri fatty acid methyl esters (FAME) into a hydroxy-free ester fraction (40-44%) consisting largely of 18∶1 (39%), 18∶2 (19%) and 18∶3 (31%), and a hydroxy ester fraction (56-60%) that was largely methyl lesquerolate (94%) with small amounts of auricolate (5%) (14-hydroxy-cis-11,cis-17-eicosadienoate) and traces of 18-carbon hydroxy esters. This process for isolating the hydroxy FAME ofLesquerella oil was scaled up 15-to 100-fold with a preparative high performance liquid chromatograph. Thirty-gram samples ofL. gordonii FAME were dissolved in eluting solvent, pumped onto the high performance liquid chromatography (HPLC) silica column and eluted with 97∶3 hexane/ethyl acetate. In an 8-hr period, up to 200 g of methyl lesquerolate could be obtained with a purity >98%, the only contaminants being methyl auricolate and methyl ricinoleate. AOCS Press, 1990 Chromatography nationallicence hydroxy acids nationallicence L. gordonii, Lesquerella fendleri nationallicence lesquerolic acid nationallicence NMR nationallicence preparative HPLC nationallicence seed oils nationallicence Carlson K. Nothern Regional Research Center, ARS/USDA, 61604, Peoria, IL aut Chaudhry A. Nothern Regional Research Center, ARS/USDA, 61604, Peoria, IL aut Peterson R. Nothern Regional Research Center, ARS/USDA, 61604, Peoria, IL aut Bagby M. Nothern Regional Research Center, ARS/USDA, 61604, Peoria, IL aut Journal of the American Oil Chemists' Society Springer-Verlag 67/8(1990-08-01), 495-498 0003-021X 67:8<495 1990 67 11746 https://doi.org/10.1007/BF02540754 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02540754 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carlson K. Nothern Regional Research Center, ARS/USDA, 61604, Peoria, IL aut NATIONALLICENCE 700 1- Chaudhry A. Nothern Regional Research Center, ARS/USDA, 61604, Peoria, IL aut NATIONALLICENCE 700 1- Peterson R. Nothern Regional Research Center, ARS/USDA, 61604, Peoria, IL aut NATIONALLICENCE 700 1- Bagby M. Nothern Regional Research Center, ARS/USDA, 61604, Peoria, IL aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/8(1990-08-01), 495-498 0003-021X 67:8<495 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer