caa a22 4500 465796818 CHVBK 20180323112040.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF02540759 doi (NATIONALLICENCE)springer-10.1007/BF02540759 Triglyceride interesterification by lipases. 1. Cocoa butter equivalents from a fraction of palm oil [Elektronische Daten] [Scott Bloomer, Patrick Adlercreutz, Bo Mattiasson] Twelve commercially available triacylglycerol lipase preparations were screened for their suitability as catalysts in the interesterification of palm oil mid fraction and ethyl stearate to form a cocoa butter equivalent. Five fungal lipase preparations were found to be suitable. The hydrolytic activity of the commercial lipase preparations was tested with sunflower seed oil and was independent of their interesterification activity. The operational stability of three of the preparations most suited for production of cocoa butter equivalents was examined. The amount of a commercial lipase preparation loaded onto a support was surveyed for optimum short-term catalytic activity. The influence of solvent concentration on the reaction rate and the purity of the product was examined at two temperatures. The optimum solvent concentration at 40°C was 1-1.5 grams of solvent/gram of substrate; at 60°C, the rate of interesterification diminished and the purity of the product decreased with increasing amounts of solvent. Four of the commercial lipase preparations found to be suitable interesterification catalysts were immobilized on five supports and their ability to catalyze the interesterification of a triglyceride and palmitic acid or ethyl palmitate was measured. The choice of support and substrate form (esterified or free fatty acid) greatly affected the catalytic activity. Some preparations were more affected by the choice of support, others by the form of the substrate. No preparation yielded maximum activity on all supports, and no support was found which produced an immobilized enzyme preparation of high activity with every commercial lipase preparation. Caution is advised in transferring observations about the suitability of a support from tests on one commerical enzyme preparation to others; individual testing is required. AOCS Press, 1990 Cocoa butter equivalent nationallicence interesterification nationallicence lipase screening nationallicence operational stability nationallicence solvent concentration nationallicence support screening nationallicence Bloomer Scott Department of Biotechnology, Chemical Center, University of Lund, P.O. Box 124, S-221 OO, Lund, Sweden aut Adlercreutz Patrick Department of Biotechnology, Chemical Center, University of Lund, P.O. Box 124, S-221 OO, Lund, Sweden aut Mattiasson Bo Department of Biotechnology, Chemical Center, University of Lund, P.O. Box 124, S-221 OO, Lund, Sweden aut Journal of the American Oil Chemists' Society Springer-Verlag 67/8(1990-08-01), 519-524 0003-021X 67:8<519 1990 67 11746 https://doi.org/10.1007/BF02540759 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02540759 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bloomer Scott Department of Biotechnology, Chemical Center, University of Lund, P.O. Box 124, S-221 OO, Lund, Sweden aut NATIONALLICENCE 700 1- Adlercreutz Patrick Department of Biotechnology, Chemical Center, University of Lund, P.O. Box 124, S-221 OO, Lund, Sweden aut NATIONALLICENCE 700 1- Mattiasson Bo Department of Biotechnology, Chemical Center, University of Lund, P.O. Box 124, S-221 OO, Lund, Sweden aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/8(1990-08-01), 519-524 0003-021X 67:8<519 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer