caa a22 4500 465797245 CHVBK 20180323112041.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF02540508 doi (NATIONALLICENCE)springer-10.1007/BF02540508 Effect of moisture, microwave heating, and live steam treatment on phospholipase D activity in soybeans and soy flakes [Elektronische Daten] [G. List, T. Mounts, A. Lanser, R. Holloway] The impact of enzyme activity on the nonhydratable phospholipid content of crude soybean oil has been evaluated. A radiochemical method was used to assay phospholipase D activity in whole and flaked soybeans stored under a variety of storage and enzyme inactivating conditions. The crude enzyme was isolated and incubated with a mixture of14C-labeled and unlabeled phosphatidylcholine. The amount of liberated radioactive choline was used as a measure of enzyme activity. whole soybeans with moisture contents of 8-18% were stored at 40°C and sampled weekly for up to four weeks. Although the enzyme was active in all samples, the optimum moisture content for enzyme activity was about 14%. Flaking and flake thickness were shown to increase phospholipas D activity. At moisture levels above 10%, flakes at .012″ showed about twice the activity of whole beans. As flake thickness was increased, enzyme activity decreased. Whole soybeans with moisture contents of 12-18% were treated by microwave heating under controlled conditions. During the early stages of heating, the enzyme was activated, and then was gradually destroyed by the time the temperature of the beans reached 115-120°C. Approximately 8-10 min of microwave heating was required to completely destroy enzymatic activity. The inactivation of phospholipase D in soyflakes treated with live steam was also evaluated. The enzyme is rapidly destroyed at temperatures of about 110°C. Evaluations of flakes subjected to live steam and whole beans treated by microwave heating to inactivate phospholipase D suggest that heat, moisture and enzyme activity are important factors contributing to the formation of nonhydratable phospholipid in extracted crude oils. AOCS Press, 1990 Degumming nationallicence microwave treatment nationallicence nonhydratable phosphatides nationallicence phospholipase D nationallicence phospholipids nationallicence phosphorus nationallicence soybean oil nationallicence steam nationallicence List G. Northern Regional Research Center. Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL aut Mounts T. Northern Regional Research Center. Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL aut Lanser A. Northern Regional Research Center. Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL aut Holloway R. Northern Regional Research Center. Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL aut Journal of the American Oil Chemists' Society Springer-Verlag 67/11(1990-11-01), 867-871 0003-021X 67:11<867 1990 67 11746 https://doi.org/10.1007/BF02540508 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02540508 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- List G. Northern Regional Research Center. Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL aut NATIONALLICENCE 700 1- Mounts T. Northern Regional Research Center. Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL aut NATIONALLICENCE 700 1- Lanser A. Northern Regional Research Center. Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL aut NATIONALLICENCE 700 1- Holloway R. Northern Regional Research Center. Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/11(1990-11-01), 867-871 0003-021X 67:11<867 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer