caa a22 4500 465797369 CHVBK 20180323112042.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF02540498 doi (NATIONALLICENCE)springer-10.1007/BF02540498 Optimization of the column loadability for the preparative HPLC Separation of soybean phospholipids [Elektronische Daten] [P. Van der Meeren, J. Vanderdeelen, M. Huys, L. Baert] A simple and rapid preparative-scale separation method was investigated in order to obtain pure soybean phospholipids. Because of technical and economical reasons, two coarse, irregular silica gels were selected. Comparing both stationary phases, a ternary mixture of hexane, 2-propanol and water yielded a different elution order of the phospholipids at analytical sample loads, in spite of the chemical similarity of these packing materials. During scaleup, it became obvious that the retention characteritics were largely influenced by the sample load, thus making the 15-35 μm RSiL inappropriate for preparative-scale separations of phospholipids. Moreover, the column loadability could be increased by controlling the flow rate. Hence, a solvent program was elaborated which enabled a column loadability of up to 2% by weight of the stationary phase. Using analytical high performance liquid chromatography, it was shown that the method proposed yielded over 90% pure phospholipids at a recovery of nearly 80%. AOCS Press, 1990 Mass detector nationallicence phosphatidylcholine nationallicence phosphatidylethanolamine nationallicence phosphatidylinositol nationallicence soybean lecithin nationallicence Van der Meeren P. Faculty of Agricultural Sciences, Department of Physical and Radiobiological Chemistry, State University of Ghent, Coupure Links 653, B-9000, Gent, Belgium aut Vanderdeelen J. Faculty of Agricultural Sciences, Department of Physical and Radiobiological Chemistry, State University of Ghent, Coupure Links 653, B-9000, Gent, Belgium aut Huys M. Faculty of Agricultural Sciences, Department of Physical and Radiobiological Chemistry, State University of Ghent, Coupure Links 653, B-9000, Gent, Belgium aut Baert L. Faculty of Agricultural Sciences, Department of Physical and Radiobiological Chemistry, State University of Ghent, Coupure Links 653, B-9000, Gent, Belgium aut Journal of the American Oil Chemists' Society Springer-Verlag 67/11(1990-11-01), 815-820 0003-021X 67:11<815 1990 67 11746 https://doi.org/10.1007/BF02540498 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02540498 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van der Meeren P. Faculty of Agricultural Sciences, Department of Physical and Radiobiological Chemistry, State University of Ghent, Coupure Links 653, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- Vanderdeelen J. Faculty of Agricultural Sciences, Department of Physical and Radiobiological Chemistry, State University of Ghent, Coupure Links 653, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- Huys M. Faculty of Agricultural Sciences, Department of Physical and Radiobiological Chemistry, State University of Ghent, Coupure Links 653, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- Baert L. Faculty of Agricultural Sciences, Department of Physical and Radiobiological Chemistry, State University of Ghent, Coupure Links 653, B-9000, Gent, Belgium aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/11(1990-11-01), 815-820 0003-021X 67:11<815 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer