caa a22 4500 465797393 CHVBK 20180323112042.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF02540486 doi (NATIONALLICENCE)springer-10.1007/BF02540486 HPLC analysis of phospholipids in crude oil for evaluation of soybean deterioration [Elektronische Daten] [T. Mounts, A. Nash] Damage to soybeans due to pre-harvest stress, storage, and export shipment has been related to an increase in the nonhydratable phospholipid content of crude oil. Phospholipids in crude soybean oil extracted from such distressed soybeans have been analyzed by gradient high-performance liquid chromatography. Crude oil was fractionated by solid phase extraction using sequential elution for recovery of phosphatides. High-performance liquid chromatography of the concentrated phospholipids was accomplished on a Lichrosorb Si-60 10 μ column, 250×4.6 mm with ultraviolet detection at 206 nm. A 20-min solvent gradient of 2-propanol/hexane/water (42∶56∶2, 51∶38∶11) gave retention profiles of phospholipid distribution (major subclasses) that changed with impact of stress applied to plant or seed. Soybeans stored at high moisture levels (16% and 20% moisture) for up to 28 days yielded oils having phosphorus contents which decreased in direct relationship to days of storage. Retention profiles were unusable for fractions isolated from oils with phosphorus content below 100 ppm. Data show that during progressive damage, the content of phosphatidylcholine and phosphatidylinositol decreased while the phosphatidic acid content increased. AOCS Press, 1990 Chromatography nationallicence crude soybean oil nationallicence degumming nationallicence enzymes nationallicence high performance liquid chromatography nationallicence oil quality nationallicence phosphatidylcholine nationallicence phosphatidylethanolamine nationallicence phosphatidylinositol nationallicence phosphatidylserine nationallicence Mounts T. Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, USDA, 61604, Peoria, IL, USA aut Nash A. Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, USDA, 61604, Peoria, IL, USA aut Journal of the American Oil Chemists' Society Springer-Verlag 67/11(1990-11-01), 757-760 0003-021X 67:11<757 1990 67 11746 https://doi.org/10.1007/BF02540486 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02540486 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mounts T. Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, USDA, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Nash A. Vegetable Oil Research, Northern Regional Research Center, Agricultural Research Service, USDA, 61604, Peoria, IL, USA aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/11(1990-11-01), 757-760 0003-021X 67:11<757 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer