caa a22 4500 465797407 CHVBK 20180323112042.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF02540488 doi (NATIONALLICENCE)springer-10.1007/BF02540488 Synthesis of phosphatidylcholine with (n−3) fatty acids by phospholipase A 2 in microemulsion [Elektronische Daten] [An Na, Caj Eriksson, Sven-Göran Eriksson, Eva Österberg, Krister Holmberg] Phosphatidylcholine containing a long chain polyunsaturated acyl group at the 2-position has been prepared by phospholipase A 2 catalyzed esterification of lysophosphatidylcholine with polyunsaturated fatty acids EPA (C20∶5) or DHA (C22∶6). Preliminary studies showed that the other fatty acids, such as lauric acid (C12), palmitic acid (C16), stearic acid (C18) and linoleic acid (C18∶2), were also incorporated. To our knowledge, phospholipase A 2 catalyzed condensation reactions have not been reported in the literature before. The reactions were performed in sodiumbis(2-ethylhexyl)-sulfosuccinate-based microemulsions containing small amounts of water. Synthesis of the same phospholipid by transesterification of phosphatidylcholine with the polyunsaturated acids in microemulsion failed; however, enzymatic hydrolysis to lysophosphatidylcholine was facile, quantitative conversion from phosphatidylcholine being attained after 16 hr reaction time. An additional observation was that, unlike enzymatic hydrolysis of phospholipids, the condensation reaction catalyzed by phospholipase A 2 was totally independent of the presence of calcium. AOCS Press, 1990 AOT nationallicence DHA nationallicence EPA nationallicence microemulsion nationallicence phosphatidylcholine nationallicence phospholipase A 2 nationallicence synthesis nationallicence Na An Department of Food Science, Chalmers University of Technology, c.o SIK, P.O. Box 5401, S-402 29, Goteborg, Sweden aut Eriksson Caj Department of Food Science, Chalmers University of Technology, c.o SIK, P.O. Box 5401, S-402 29, Goteborg, Sweden aut Eriksson Sven-Göran SIK-The Swedish Institute for Food Research, S-444 85, Stenungsund, Sweden aut Österberg Eva Berol Nobel AB, S-444 85, Stenungsund, Sweden aut Holmberg Krister Berol Nobel AB, S-444 85, Stenungsund, Sweden aut Journal of the American Oil Chemists' Society Springer-Verlag 67/11(1990-11-01), 766-770 0003-021X 67:11<766 1990 67 11746 https://doi.org/10.1007/BF02540488 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02540488 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Na An Department of Food Science, Chalmers University of Technology, c.o SIK, P.O. Box 5401, S-402 29, Goteborg, Sweden aut NATIONALLICENCE 700 1- Eriksson Caj Department of Food Science, Chalmers University of Technology, c.o SIK, P.O. Box 5401, S-402 29, Goteborg, Sweden aut NATIONALLICENCE 700 1- Eriksson Sven-Göran SIK-The Swedish Institute for Food Research, S-444 85, Stenungsund, Sweden aut NATIONALLICENCE 700 1- Österberg Eva Berol Nobel AB, S-444 85, Stenungsund, Sweden aut NATIONALLICENCE 700 1- Holmberg Krister Berol Nobel AB, S-444 85, Stenungsund, Sweden aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/11(1990-11-01), 766-770 0003-021X 67:11<766 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer