caa a22 4500 465797504 CHVBK 20180323112042.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF02540504 doi (NATIONALLICENCE)springer-10.1007/BF02540504 Increase in the γ-linolenic acid content by solvent winterization of fungal oil extracted from Mortierella genus [Elektronische Daten] [T. Yokochi, M. Usita, Y. Kamisaka, T. Nakahara, O. Suzuki] The fungal oil extracted fromMortierella ramanniana var.angulispora (IFO 8187) was solvent winterized in order to raise the content of γ-linolenic acid (GLA). Effects of winterization conditions (solvent, oil concentration in the solvent and temperature) and changes of glyceride compositions were discussed. The fungal oil was separated into four diglycerides and 17 triglycerides (TG) with high performance liquid chromatography. The predominant species were POO, POP and LOP, whose contents were 24.4, 22.9 and 9.4% of the total TG, respectively. Ethanol at 4°C gave the highest GLA content of 10.5% in spite of lower yield than with acetone at −20°C. The highest separation efficiency for GLA (ηGLA) was 0.27 with acetone at −20°C and 10% oil concentration, resulting in 8.3% of GLA from the fungal oil at 5.7% LGA. In case of lower oil concentration at 5-20%, ηGLA showed higher in the following order: acetone (−20°C)>n-hexane (−20°C)>acetone (4°C)>petroleum ether (−20°C). The winterization process also proved to be effective for the separation of TG type, Sa2U (Sa; saturated fatty acid; U, unsaturated fatty acid) into the crystallized fraction and SaU2 into the liquid fraction. Acetone at −20°C showed higher separation efficiency for triunsaturated TG than the other solvents. AOCS Press, 1990 Crystallization nationallicence fungal oil nationallicence glyceride composition nationallicence linolenic acid (GLA) nationallicence Mortierella ramanniana nationallicence separation efficiency nationallicence Triglyceride composition nationallicence winterization nationallicence Yokochi T. National Chemical Laboratory for Industry, 1-1, Higashi, Tsukuba, Ibaraki, Japan aut Usita M. National Institute of Science and Technology, National Science and Technology Authority, Manila, Philippines aut Kamisaka Y. National Chemical Laboratory for Industry, 1-1, Higashi, Tsukuba, Ibaraki, Japan aut Nakahara T. National Chemical Laboratory for Industry, 1-1, Higashi, Tsukuba, Ibaraki, Japan aut Suzuki O. National Chemical Laboratory for Industry, 1-1, Higashi, Tsukuba, Ibaraki, Japan aut Journal of the American Oil Chemists' Society Springer-Verlag 67/11(1990-11-01), 846-851 0003-021X 67:11<846 1990 67 11746 https://doi.org/10.1007/BF02540504 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02540504 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yokochi T. National Chemical Laboratory for Industry, 1-1, Higashi, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Usita M. National Institute of Science and Technology, National Science and Technology Authority, Manila, Philippines aut NATIONALLICENCE 700 1- Kamisaka Y. National Chemical Laboratory for Industry, 1-1, Higashi, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Nakahara T. National Chemical Laboratory for Industry, 1-1, Higashi, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Suzuki O. National Chemical Laboratory for Industry, 1-1, Higashi, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Journal of the American Oil Chemists' Society Springer-Verlag 67/11(1990-11-01), 846-851 0003-021X 67:11<846 1990 67 11746 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer