caa a22 4500 465803717 CHVBK 20180323112058.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF03348580 doi (NATIONALLICENCE)springer-10.1007/BF03348580 Bromocriptine reduces rat thyrotropin β-subunit mRNA stability [Elektronische Daten] [A. Levy, S. Lightman] We have examined the effect of orally administered bromocriptine on TSHβ- subunit messenger (m)RNA in the anterior pituitary glands of Sprague-Dawley rats using in situ and dot-blot hybridization histochemistry. Quantitative in situ hybridization of pituitary sections demonstrated a 60% reduction in TSHβ- subunit mRNA probe binding from rats fed a diet containing bromocriptine 10 mg/kg/day. This was confirmed by dot-blot analysis of nuclear and cytoplasmic pituitary extracts from the same tissue. Hybridization of cytoplasmic extracts of pituitary cells cultured under actinomycin D-induced transcription arrest showed that part of the effect of bromocriptine appeared to be mediated through a change in TSHβ-subunit mRNA stability and implies that the acute influence of dopamine on TSH metabolism may be transduced by control of TSHβ-subunit mRNA catabolism. This suggests a mechanism by which cells with relatively stable tissue specific mRNAs appear to respond rapidly to hormonal effects at the transcriptional level. Italian Society of Endocrinology (SIE), 1990 Anterior pituitary nationallicence thyrotropin mRNA stability nationallicence hybridization nationallicence Northern analysis nationallicence Levy A. The Medical Unit, Charing Cross and Westminster Medical School, 17 Page Street, London, SW1P 2AP, England aut Lightman S. The Medical Unit, Charing Cross and Westminster Medical School, 17 Page Street, London, SW1P 2AP, England aut Journal of Endocrinological Investigation Springer International Publishing 13/1(1990-01-01), 49-53 0391-4097 13:1<49 1990 13 40618 https://doi.org/10.1007/BF03348580 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03348580 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Levy A. The Medical Unit, Charing Cross and Westminster Medical School, 17 Page Street, London, SW1P 2AP, England aut NATIONALLICENCE 700 1- Lightman S. The Medical Unit, Charing Cross and Westminster Medical School, 17 Page Street, London, SW1P 2AP, England aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/1(1990-01-01), 49-53 0391-4097 13:1<49 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer