caa a22 4500 465803911 CHVBK 20180323112059.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF03348604 doi (NATIONALLICENCE)springer-10.1007/BF03348604 Analysis of the hypothalamic-pituitary-ovary axis in the neonatally-androgenized female rat [Elektronische Daten] [Eduardo Spinedi, V. Mariani, M. Bulfon, M. Colombani-Vidal, H. Scaglia] The administration of testosterone propionate (TP) in the female rat at the neonatal age has been used for several yr as a model to study anovulation during adulthood. The present work was designed in order to see whether some neuroendocrine parameters vary with age in this animal model. Hypothalamic LHRH content and LH-FSH anterior pituitary (AP) content and plasma levels were evaluated in samples taken from both neonatally-androgenized and littermate control female rats at different ages (15 to 100 days old). Additionally, we have studied pulsatile LH-FSH released in plasma and in vivo AP response to LHRH in both neonatally-androgenized and control female rats during adulthood. The results indicate that the neonatal TP treatment did not induce any change in hypothalamic LHRH content over development. Neonatally androgenized rats have decreased both LH-FSH AP content and plasma levels at the infantile age (15-day old). LH-FSH AP content remained reduced in samples taken up to the 30th day of age. Plasma LH-FSH levels on the day 30 of age were similar in both groups. TP-treated rats studied on the 100th day of age had: a) an altered pulsatile rhythm of gonadotropin release in plasma due to the decreased LH-FSH trough and average mean values, and to the diminished FSH peak amplitude values, as well as an increased LH:FSH ratio; and b) an impaired in vivo LHRH-induced LH-FSH release. In summary, the present study indicates that after the neonatal treatment with TP: 1) a reduction on both LH-FSH AP content and plasma levels is established at the infantile age, 2) a decreased pattern of pulsatile LH-FSH secretion in plasma is seen during adulthood, which is probably due, at least in part, to a decreased AP response to LHRH stimulation, and 3) although the synthesis of hypothalamic LHRH had not been altered over development, a modification of median eminence LHRH secretion into the portal blood must be not excluded. These results further suggest that the neonatal administration of TP could induce permanent sterility in the female rat by altering the AP function. Whether this treatment also alters other peripheral glands (ovary and/or adrenal) still remains unclear. Italian Society of Endocrinology (SIE), 1990 LHRH nationallicence LH-FSH nationallicence androgenized rats nationallicence Spinedi Eduardo Centro de Referencia de Radioinmunoensayo, Centro de Especialistas en Analisis Biologicos Distrito I., calle 47 no. 1015, 1900, La Plata, Argentina aut Mariani V. Centro de Referencia de Radioinmunoensayo, Centro de Especialistas en Analisis Biologicos Distrito I., calle 47 no. 1015, 1900, La Plata, Argentina aut Bulfon M. Centro de Referencia de Radioinmunoensayo, Centro de Especialistas en Analisis Biologicos Distrito I., calle 47 no. 1015, 1900, La Plata, Argentina aut Colombani-Vidal M. Centro de Referencia de Radioinmunoensayo, Centro de Especialistas en Analisis Biologicos Distrito I., calle 47 no. 1015, 1900, La Plata, Argentina aut Scaglia H. Centro de Referencia de Radioinmunoensayo, Centro de Especialistas en Analisis Biologicos Distrito I., calle 47 no. 1015, 1900, La Plata, Argentina aut Journal of Endocrinological Investigation Springer International Publishing 13/6(1990-06-01), 481-488 0391-4097 13:6<481 1990 13 40618 https://doi.org/10.1007/BF03348604 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03348604 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Spinedi Eduardo Centro de Referencia de Radioinmunoensayo, Centro de Especialistas en Analisis Biologicos Distrito I., calle 47 no. 1015, 1900, La Plata, Argentina aut NATIONALLICENCE 700 1- Mariani V. Centro de Referencia de Radioinmunoensayo, Centro de Especialistas en Analisis Biologicos Distrito I., calle 47 no. 1015, 1900, La Plata, Argentina aut NATIONALLICENCE 700 1- Bulfon M. Centro de Referencia de Radioinmunoensayo, Centro de Especialistas en Analisis Biologicos Distrito I., calle 47 no. 1015, 1900, La Plata, Argentina aut NATIONALLICENCE 700 1- Colombani-Vidal M. Centro de Referencia de Radioinmunoensayo, Centro de Especialistas en Analisis Biologicos Distrito I., calle 47 no. 1015, 1900, La Plata, Argentina aut NATIONALLICENCE 700 1- Scaglia H. Centro de Referencia de Radioinmunoensayo, Centro de Especialistas en Analisis Biologicos Distrito I., calle 47 no. 1015, 1900, La Plata, Argentina aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/6(1990-06-01), 481-488 0391-4097 13:6<481 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer