caa a22 4500 465803970 CHVBK 20180323112059.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF03348602 doi (NATIONALLICENCE)springer-10.1007/BF03348602 Effects of active immunization against L-triiodothyronine on serum thyrotropin levels and liver mitochondrial α-glycerophosphate dehydrogenase activity in rats: evidence for reduced hormone disposal to cells [Elektronische Daten] [G. Costante, D. Crupi, R. Catalfamo, F. Trimarchi] Thyroid hormone binding autoantibodies have been observed in serum from patients affected by either thyroid disorders or autoimmune nonthyroid diseases.ln most instances, no major involvement of thyroid status of these patients has been reported. However, some authors have attributed the occurrence of hypothyroidism to high capacity thyroid hormone binding autoantibodies.In order to verify such a hypothesis, the effect of these autoantibodies on serum TSH and liver mitochondrial alpha-glycerophosphate dehydrogenase has been investigated in rats, wherein circulating T3-binding antibodies (max cap10−12 10−8 to mol/l) were induced by active immunization.Starting from the 3rd week after antigen injection, the binding of 125I-T3 to serum immunoglobulins was progressively increased, with a peak at the 5th week. In immunized animals considered as a whole group, serum TSH levels were significantly higher and liver mitochondrial alpha-glycerophosphate dehydrogenase activity was significantly lower than in normal rats (m ± SE: 145 ± 15 vs 34 ± 2 ng/dl and 0.0450 ± 008 vs 0.0980 ± 005 ΔA. mg/prot/.min, respectively; p < 0.001) The pattern of both TSH increase and alpha-glycerophosphate dehydrogenase decrease were similar to the evolution of 125-I-T3 binding to serum immunoglobulins, the maximal TSH values and lowest enzyme levels being observed at the 5th week after the beginning of the experiment. These results indicate that high capacity thyroid hormone binding autoantibodies may actually reduce thyroid hormone disposal at cellular level, at least prior to the attainment of steady state T3- antibody hormone binding. Italian Society of Endocrinology (SIE), 1990 Thyroid hormone binding proteins nationallicence thyroid hormone autoantibodies nationallicence rat TSH nationallicence liver mitochondrial nationallicence GPD nationallicence hypothyroidism nationallicence Costante G. Istituto Pluridisciplinare di Clinica Medica e Terapia Medica, University of Messina, Policlinico Universitario, 98125, Messina, Italy aut Crupi D. Istituto Pluridisciplinare di Clinica Medica e Terapia Medica, University of Messina, Policlinico Universitario, 98125, Messina, Italy aut Catalfamo R. Istituto Pluridisciplinare di Clinica Medica e Terapia Medica, University of Messina, Policlinico Universitario, 98125, Messina, Italy aut Trimarchi F. Istituto Pluridisciplinare di Clinica Medica e Terapia Medica, University of Messina, Policlinico Universitario, 98125, Messina, Italy aut Journal of Endocrinological Investigation Springer International Publishing 13/6(1990-06-01), 469-474 0391-4097 13:6<469 1990 13 40618 https://doi.org/10.1007/BF03348602 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03348602 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Costante G. Istituto Pluridisciplinare di Clinica Medica e Terapia Medica, University of Messina, Policlinico Universitario, 98125, Messina, Italy aut NATIONALLICENCE 700 1- Crupi D. Istituto Pluridisciplinare di Clinica Medica e Terapia Medica, University of Messina, Policlinico Universitario, 98125, Messina, Italy aut NATIONALLICENCE 700 1- Catalfamo R. Istituto Pluridisciplinare di Clinica Medica e Terapia Medica, University of Messina, Policlinico Universitario, 98125, Messina, Italy aut NATIONALLICENCE 700 1- Trimarchi F. Istituto Pluridisciplinare di Clinica Medica e Terapia Medica, University of Messina, Policlinico Universitario, 98125, Messina, Italy aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/6(1990-06-01), 469-474 0391-4097 13:6<469 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer