caa a22 4500 465804292 CHVBK 20180323112059.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF03348626 doi (NATIONALLICENCE)springer-10.1007/BF03348626 Quantification of intracellular calcitonin gene transcripts in human medullary thyroid carcinoma (MTC) by in situ hybridization [Elektronische Daten] [Michèle Noel, A. Gavoille, F. Lasmoles, E. Kahn, B. Caillou, P. Gardet, P. Fragu] The human calcitonin gene generates 2 distinct mature mRNAs by alternative RNA processing, encoding calcitonin (CT) in thyroid C-cells or a neuropeptide (CGRP) in the brain. We evaluated quantitatively by in situ hybridization the expression of the CT gene in tissue section of 5 MTCs (2 sporadic and 3 familial forms). The primary tumor of one MTC was compared to a brain metastasis. In situ hybridization was carried out with tritiated cDNA probes coding for CT and CGRP mRNA. After autoradiography the number of silver grains was conunted in 400 cells by computerized analysis of digitized images and expressed as the labelling level (L.L. = grain area/cell area per day of autoradiographic exposure). This was used to calculate the relative abundance per cell of the specific messengers studied, which depends on the autoradiographic efficiency and the specific activity of the probe used. The CT mRNA content was 3.25-6.55 10−10 μg equivalents in the 3 familial forms of MTC and 4.95-9.25 10−10 μg equivalents for the 2 sporadic forms. The levels of CT mRNA in the brain metastasis and in the primary tumor were identical (4.10 10−10 μg equivalents). CGRP mRNA expression was weaker in the sporadic and in the familial thyroid tumors (0.60-1.65 10−10 μg equivalents). The content of mRNA CGRP in the brain metastasis (0.60 10~10 /xg equivalents) was lower than that in the primary tumor (1.05 10−10 μg equivalents). These results suggest that most of the primary RNA transcript generated by CT gene lead to the formation of CT mRNA in MTC, and the alternative mRNA processing pathway in sporadic and familial forms does not seem to be different. No correlation was found between the amount of tumor CT mRNA and clinical course of the MTC. The latter appears to be more linked to the initial extent of the disease. Italian Society of Endocrinology (SIE), 1990 In situ hybridization nationallicence medullary thyroid carcinoma nationallicence calcitonin gene expression nationallicence CT mRNA nationallicence CGRP mRNA nationallicence image analysis nationallicence grain counting nationallicence Noel Michèle Inserm U66, C.H.U. Saint-Antoine, Paris, France aut Gavoille A. Inserm U66, C.H.U. Saint-Antoine, Paris, France aut Lasmoles F. Inserm U113, C.H.U. Saint-Antoine, Paris, France aut Kahn E. Inserm U66, C.H.U. Saint-Antoine, Paris, France aut Caillou B. Histopathologie D, C.H.U. Saint-Antoine, Paris, France aut Gardet P. Unité de Medecine Nucléaire, Institut Gustave-Roussy, Villejuif, C.H.U. Saint-Antoine, Paris, France aut Fragu P. Inserm U66, C.H.U. Saint-Antoine, Paris, France aut Journal of Endocrinological Investigation Springer International Publishing 13/7(1990-07-01), 567-573 0391-4097 13:7<567 1990 13 40618 https://doi.org/10.1007/BF03348626 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03348626 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Noel Michèle Inserm U66, C.H.U. Saint-Antoine, Paris, France aut NATIONALLICENCE 700 1- Gavoille A. Inserm U66, C.H.U. Saint-Antoine, Paris, France aut NATIONALLICENCE 700 1- Lasmoles F. Inserm U113, C.H.U. Saint-Antoine, Paris, France aut NATIONALLICENCE 700 1- Kahn E. Inserm U66, C.H.U. Saint-Antoine, Paris, France aut NATIONALLICENCE 700 1- Caillou B. Histopathologie D, C.H.U. Saint-Antoine, Paris, France aut NATIONALLICENCE 700 1- Gardet P. Unité de Medecine Nucléaire, Institut Gustave-Roussy, Villejuif, C.H.U. Saint-Antoine, Paris, France aut NATIONALLICENCE 700 1- Fragu P. Inserm U66, C.H.U. Saint-Antoine, Paris, France aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/7(1990-07-01), 567-573 0391-4097 13:7<567 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer