caa a22 4500 465804500 CHVBK 20180323112100.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF03349546 doi (NATIONALLICENCE)springer-10.1007/BF03349546 Luteinizing hormone determinations obtained with either a monoclonal and a polyclonal antibody radioimmunoassay and their correlations with clinical findings [Elektronische Daten] [P. Venturini, V. Remorgida, V. Aguggia, L. De Cecco] Detection of circulating levels of gonadotropin is fundamental in reproductive physiology and related disorders. The advent of monoclonal antibodies might supply the clinician with faster and more accurate estimates of these hormones. We have evaluated the hormonal determinations provided by a monoclonal assay and those generated from our previous assay, produced by the same manufacturer, based on polyclonal antibodies. Comparison was performed on blood samples collected from volunteers with regular menstruation (n = 51) or patients attending our Clinic. The correlation between data provided by the two assays fluctuated from the almost complete overlap of the physiological menstrual cycle to the striking dissociation of the patients (n = 13) receiving a Gonadotropin Hormone-Releasing Hormone analog for gonadotropin suppression. Intermediate coefficients of correlations were recorded in normal subjects undergoing a Gonadotropin Hormone-Releasing Hormone stimulation test (n = 12) and in patients with functional hypothalamic amenorrhea (n = 19) or polycystic ovarian disease (n = 37). Values obtained with the monoclonal assay tended to be lower than those recorded with the previous system. A closer correlation with the clinical findings was observed in the hormonal values originated with the monoclonal assay. Italian Society of Endocrinology (SIE), 1990 Radioimmunoassay nationallicence immunoradiometric nationallicence gonadotropin nationallicence detection nationallicence monoclonal antibody nationallicence Venturini P. Clinica Ostetrica e Ginecologica, Universitàdi Genova, Ospedale San Martino, Padiglione 1, Viale Benedetto XV, 16132, Genova, Italy aut Remorgida V. Clinica Ostetrica e Ginecologica, Universitàdi Genova, Ospedale San Martino, Padiglione 1, Viale Benedetto XV, 16132, Genova, Italy aut Aguggia V. Clinica Ostetrica e Ginecologica, Universitàdi Genova, Ospedale San Martino, Padiglione 1, Viale Benedetto XV, 16132, Genova, Italy aut De Cecco L. Clinica Ostetrica e Ginecologica, Universitàdi Genova, Ospedale San Martino, Padiglione 1, Viale Benedetto XV, 16132, Genova, Italy aut Journal of Endocrinological Investigation Springer International Publishing 13/3(1990-03-01), 227-234 0391-4097 13:3<227 1990 13 40618 https://doi.org/10.1007/BF03349546 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03349546 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Venturini P. Clinica Ostetrica e Ginecologica, Universitàdi Genova, Ospedale San Martino, Padiglione 1, Viale Benedetto XV, 16132, Genova, Italy aut NATIONALLICENCE 700 1- Remorgida V. Clinica Ostetrica e Ginecologica, Universitàdi Genova, Ospedale San Martino, Padiglione 1, Viale Benedetto XV, 16132, Genova, Italy aut NATIONALLICENCE 700 1- Aguggia V. Clinica Ostetrica e Ginecologica, Universitàdi Genova, Ospedale San Martino, Padiglione 1, Viale Benedetto XV, 16132, Genova, Italy aut NATIONALLICENCE 700 1- De Cecco L. Clinica Ostetrica e Ginecologica, Universitàdi Genova, Ospedale San Martino, Padiglione 1, Viale Benedetto XV, 16132, Genova, Italy aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/3(1990-03-01), 227-234 0391-4097 13:3<227 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer