caa a22 4500 465804616 CHVBK 20180323112100.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF03349542 doi (NATIONALLICENCE)springer-10.1007/BF03349542 Plasma clearance of heterogeneous growth hormone components in the rat: effects of diabetes and starvation [Elektronische Daten] [T. Jolin, C. González, Margarita González] Two forms of immunoreactive rat GH (rGH), big rGH (b · rGH) and little rGH (I · rGH) are predominant in both pituitary and plasma from control (C), fasted (F), food-restricted (FR), diabetic (D) and insulin-treated D (D + I) rats. The I · rGH appears to be the predominant form in plasma and pituitary in all five experimental groups. However, in each group, the relative proportion of b · rGH in plasma exceeds from that in pituitary. To determine whether these differences are due to a delayed metabolic clearance of the big component, the MCRS and distribution volume of b · rGH, I · rGH and total rGH were measured in C, F, FR, D and D +1 rats. Pituitary extracts, b · rGH and I · rGH were injected as a bolus, and the disappearance of each component was followed by RIA. In each group, the MCR of the big form was significantly lower than that of total rGH and I · rGH. Moreover, MCRS of total rGH, b · rGH and I · rGH in F and D rats were lower than that of the corresponding component in C animals. The decreased b · rGH MCR in each experimental group, and that of total rGH, b · rGH and I · rGH in F and D animals, is associated with a lower distribution space for the above cited components. Furthermore, the reduced receptor-binding activities for the larger form of rGH, and the loss of hepatic GH receptors in D rats, together with the possibility that b · rGH could be presumably excluded from the glomerular filtration and subsequent degradation, may be reasons for the longer persistence of b rGH in plasma in each experimental group, and for prolonged permanence of I · rGH in plasma in F and D rats. Italian Society of Endocrinology (SIE), 1990 Diabetes nationallicence starvation nationallicence food-restriction nationallicence big rGH MCR nationallicence little rGH MCR nationallicence Jolin T. Instituto de Investigaciones Biomédicas del C.S.I.C., Facultad de Medicina, Universidad Autónoma de Madrid, C/Arzobispo Morcillo 4, 28029, Madrid, Spain aut González C. Instituto de Investigaciones Biomédicas del C.S.I.C., Facultad de Medicina, Universidad Autónoma de Madrid, C/Arzobispo Morcillo 4, 28029, Madrid, Spain aut González Margarita Instituto de Investigaciones Biomédicas del C.S.I.C., Facultad de Medicina, Universidad Autónoma de Madrid, C/Arzobispo Morcillo 4, 28029, Madrid, Spain aut Journal of Endocrinological Investigation Springer International Publishing 13/3(1990-03-01), 209-216 0391-4097 13:3<209 1990 13 40618 https://doi.org/10.1007/BF03349542 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03349542 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jolin T. Instituto de Investigaciones Biomédicas del C.S.I.C., Facultad de Medicina, Universidad Autónoma de Madrid, C/Arzobispo Morcillo 4, 28029, Madrid, Spain aut NATIONALLICENCE 700 1- González C. Instituto de Investigaciones Biomédicas del C.S.I.C., Facultad de Medicina, Universidad Autónoma de Madrid, C/Arzobispo Morcillo 4, 28029, Madrid, Spain aut NATIONALLICENCE 700 1- González Margarita Instituto de Investigaciones Biomédicas del C.S.I.C., Facultad de Medicina, Universidad Autónoma de Madrid, C/Arzobispo Morcillo 4, 28029, Madrid, Spain aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/3(1990-03-01), 209-216 0391-4097 13:3<209 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer