caa a22 4500 465804837 CHVBK 20180323112101.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF03349621 doi (NATIONALLICENCE)springer-10.1007/BF03349621 Pulsatile secretion of luteinizing hormone in agonadal men before and during testosterone replacement therapy [Elektronische Daten] [A. Genazzani, G. Forti, M. Maggi, M. Milloni, F. Cianfanelli, V. Guardabasso, V. Toscano, M. Serio, D. Rodbard] We have reevaluated the question regarding the pulsatile pattern of LH secretion in agonadal men before and following testosterone replacement therapy. Five normal males were used as a reference group and four agonadal men were studied before and during replacement therapy with testosterone enanthate. All the subjects were sampled every 5 min for 12 h (08:00 to 20:00). Data were analyzed using the statistically based and validated pulse detection program DETECT. The normal subjects showed an LH pulse frequency of 10.2 ± 1.7 peaks/12 h (mean ± SEM) and a mean duration of 48.8 ± 14 min, while in agonadal patients without testosterone replacement the frequency of LH peaks (27.5 ± 2 peaks/12 h) was significantly higher than for normal subjects (p < 0.05), and the mean duration of peaks was lower than in controls (17.2 ± 1.2 min; p < 0.01). Following chronic testosterone enanthate replacement therapy (200 mg im every two weeks) these patients showed an increase in the duration and a significant reduction in the frequency of LH peaks (from 27.5 ± 2 to 18.2 ± 2.1 peaks/12 h; p < 0.01) but pulse frequency remained significantly higher than for normal subjects (p < 0.01). This finding is independent of the choice of p values for false positive detection rate (p = 0.01 or p =0.005), but it does depend on sampling frequency and is influenced by large (four-fold) changes in the thresholds for peak detection. Using a "discrete deconvolution” technique we estimated the instantaneous secretory rate (ISR) for the two groups of patients. The results using ISR corroborated the findings obtained using analysis of observed plasma LH measurements. ISR computation also showed that the duration of the secretory events of the gonadotropes is significantly shorter (p < 0.01) than the one estimated on plasma concen-tration, both in normal subjects and in agonadal patients before and during testosterone administration. In conclusion: LH pulse frequency observed in basal conditions in agonadal men was much higher than previously reported in primary testicular failure; during conventional testosterone replacement therapy LH pulse frequency of agonadal men was significantly reduced but still higher (p < 0.01) than in normal men. This finding is probably related to the subnormal plasma levels of testosterone found in agonadal men during the replacement therapy; the analysis of data using a sampling interval of 10 min gave results similar to previous reports, confirming that the choice of sampling interval can markedly affect the evaluation of frequent LH pulsatile secretion. Italian Society of Endocrinology (SIE), 1990 Genazzani A. Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA aut Forti G. Endocrinology Unit, Università di Firenze, Viale Pieraccini 6, 50134, Firenze, Italy aut Maggi M. Endocrinology Unit, Università di Firenze, Viale Pieraccini 6, 50134, Firenze, Italy aut Milloni M. Endocrinology Unit, Università di Firenze, Viale Pieraccini 6, 50134, Firenze, Italy aut Cianfanelli F. Endocrinology Unit, Università di Firenze, Viale Pieraccini 6, 50134, Firenze, Italy aut Guardabasso V. Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, S. Maria Imbaro (Chieti), Italy aut Toscano V. Clinica Medica V, Università La Sapienza, Roma, Italy aut Serio M. Endocrinology Unit, Università di Firenze, Viale Pieraccini 6, 50134, Firenze, Italy aut Rodbard D. Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA aut Journal of Endocrinological Investigation Springer International Publishing 13/10(1990-11-01), 777-786 0391-4097 13:10<777 1990 13 40618 https://doi.org/10.1007/BF03349621 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03349621 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Genazzani A. Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Forti G. Endocrinology Unit, Università di Firenze, Viale Pieraccini 6, 50134, Firenze, Italy aut NATIONALLICENCE 700 1- Maggi M. Endocrinology Unit, Università di Firenze, Viale Pieraccini 6, 50134, Firenze, Italy aut NATIONALLICENCE 700 1- Milloni M. Endocrinology Unit, Università di Firenze, Viale Pieraccini 6, 50134, Firenze, Italy aut NATIONALLICENCE 700 1- Cianfanelli F. Endocrinology Unit, Università di Firenze, Viale Pieraccini 6, 50134, Firenze, Italy aut NATIONALLICENCE 700 1- Guardabasso V. Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, S. Maria Imbaro (Chieti), Italy aut NATIONALLICENCE 700 1- Toscano V. Clinica Medica V, Università La Sapienza, Roma, Italy aut NATIONALLICENCE 700 1- Serio M. Endocrinology Unit, Università di Firenze, Viale Pieraccini 6, 50134, Firenze, Italy aut NATIONALLICENCE 700 1- Rodbard D. Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/10(1990-11-01), 777-786 0391-4097 13:10<777 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer