caa a22 4500 465805302 CHVBK 20180323112102.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF03350684 doi (NATIONALLICENCE)springer-10.1007/BF03350684 Changes in adrenal neuropeptides content [peptide 7B2, neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP)] induced by pharmacological and hormonal manipulations [Elektronische Daten] [T. Tsuchiya, Y. Suzuki, H. Suzuki, R. Ohtake, S-I. Shimoda] A novel pituitary peptide, designated 7B2, was shown to be present in the adrenal gland. 7B2-like immunoreactivity was mainly localized in the adrenal medulla, similarly to NPY and VIP. In order to elucidate the neural and humoral regulation of adrenal 7B2, NPY and VIP content, Wister rats were treated with reserpine (RES), ranitidine (RANT) or chlorpheniramine maleate (CPhM) for 7-10 days. The thyroid hormone excess and deficient states were experimentally produced with thyroxine (T4) treatment for 2 weeks, methylmer-captoimidazole (MMI) for 4 weeks, or a thyroidectomized state (Tx) for 4 weeks. Orchiectomy or neonatal monosodium glutamate (MSG) treatment was also done. 7B2, NPY and VIP contents were measured by specific radioimmunoassays. RES and RANT treatments caused significant 7B2 reduction (p < 0.01) and adrenal NPY was significantly decreased by RES (p < 0.05), while CPhM induced a VIP decreased (p < 0.05). Orchiectomy did not affect the peptides concentrations, though MSG treatment did cause a reverse change in VIP and NPY. Although T4 administration did not cause any significant change, MMI treatment and Tx induced significant increase (p < 0.05 or p < 0.01) in these peptides. Gel or high performance liquid chromatographic analysis revealed the majority of each immunoreactivity coeluted with each standard. These results suggested that adrenal NPY seemed to be coregulated with catecholamine, while VIP was mainly affected by histaminergic control. Furthermore 7B2 might be modulated by both catecholaminergic and histaminergic nervous control. Thyroid hormone deficiency may also affect the amount of these peptides. Italian Society of Endocrinology (SIE), 1990 Peptide 7B2 nationallicence neuropeptide Y nationallicence vasoactive intestinal polypeptide nationallicence adrenal gland nationallicence Tsuchiya T. Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan aut Suzuki Y. Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan aut Suzuki H. Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan aut Ohtake R. Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan aut Shimoda S-I Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan aut Journal of Endocrinological Investigation Springer International Publishing 13/5(1990-05-01), 381-389 0391-4097 13:5<381 1990 13 40618 https://doi.org/10.1007/BF03350684 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03350684 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsuchiya T. Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan aut NATIONALLICENCE 700 1- Suzuki Y. Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan aut NATIONALLICENCE 700 1- Suzuki H. Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan aut NATIONALLICENCE 700 1- Ohtake R. Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan aut NATIONALLICENCE 700 1- Shimoda S-I Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/5(1990-05-01), 381-389 0391-4097 13:5<381 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer