caa a22 4500 465805418 CHVBK 20180323112102.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF03350686 doi (NATIONALLICENCE)springer-10.1007/BF03350686 Effects of vitamin D3metabolites on thyrotropin secretion from rat pituitary cells in culture [Elektronische Daten] [K. Törnquist, L. Forss, C. Lamberg-Allardt] The effects of several vitamin D3 metabolites on TSH secretion were investigated using rat pituitary cells in culture. After a 24-h incubation in Dulbecco's medium, only 25,26-dihydroxyvitamin D3 [25,26(OH)2D3] at 10-6 M increased the basal TSH secretion (36% above control). The TRH-induced TSH secretion increased dose-dependently with increasing concentrations of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] showing a significant increase at 10-8 M (41% above control). At 10-5 M 25,26(OH)2D3 the TRH-stimulated TSH secretion was also increased (63% above control). In medium containing charcoal-treated serum, 1, 24,25-trihydroxyvitamin D3 [1,24,25(OH)3D3] decreased the TRH-induced TSH secretion dose dependency (34% below control at 10-8 M), while the effects of both 1,25(OH)2D3 and 25,26(OH)2D3 were blunted. Our results indicate that several vitamin D3 metabolites may affect pituitary TSH secretion. Italian Society of Endocrinology (SIE), 1990 Vitamin D3 metabolites nationallicence TSH nationallicence TRH nationallicence pituitary cells nationallicence Törnquist K. Endocrine Research Laboratory, University of Helsinki, Minerva Foundation, Institute tor Medical Research, P.O. Box 819, 00101, Helsinki, Finland aut Forss L. Endocrine Research Laboratory, University of Helsinki, Minerva Foundation, Institute tor Medical Research, P.O. Box 819, 00101, Helsinki, Finland aut Lamberg-Allardt C. Endocrine Research Laboratory, University of Helsinki, Minerva Foundation, Institute tor Medical Research, P.O. Box 819, 00101, Helsinki, Finland aut Journal of Endocrinological Investigation Springer International Publishing 13/5(1990-05-01), 391-395 0391-4097 13:5<391 1990 13 40618 https://doi.org/10.1007/BF03350686 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03350686 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Törnquist K. Endocrine Research Laboratory, University of Helsinki, Minerva Foundation, Institute tor Medical Research, P.O. Box 819, 00101, Helsinki, Finland aut NATIONALLICENCE 700 1- Forss L. Endocrine Research Laboratory, University of Helsinki, Minerva Foundation, Institute tor Medical Research, P.O. Box 819, 00101, Helsinki, Finland aut NATIONALLICENCE 700 1- Lamberg-Allardt C. Endocrine Research Laboratory, University of Helsinki, Minerva Foundation, Institute tor Medical Research, P.O. Box 819, 00101, Helsinki, Finland aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/5(1990-05-01), 391-395 0391-4097 13:5<391 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer