caa a22 4500 465805426 CHVBK 20180323112102.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF03350689 doi (NATIONALLICENCE)springer-10.1007/BF03350689 Effect of thyrotropin and cAMP on FRTL5 cell growth in a serum free medium [Elektronische Daten] [G. Damante, D. Russo, D. Foti, G. Grasso, S. Filetti] TSH-induced proliferation of FRTL5 cells was studied in a chemically serum-free, defined medium. FRTL5 cells incubated for several days in a medium lacking of serum and hormones were fully able to respond to TSH in terms of cAMP production and iodine uptake. In the same medium, TSH stimulated FRTL5 cell growth as assessed by thymidine incorporation, DNA content and cell count. In these experimental conditions the cellular doubling time was of 7.5 days as compared to 40 h when calf serum was present together with TSH. Cholera toxin, forskolin and (bu)2cAMP, substances able to selectively increase intracellular cAMP levels, were not as efficient as TSH in inducing FRTL5 cell growth. However, both TSH and (bu)2cAMP, in a similar way, induced c-myc gene expression and cellular progression through the prereplicative phase of cell division. These data demonstrate that cAMP-dependent mechanisms are only partially responsible for TSH-induced FRTL5 cell growth, thus suggesting a role of cAMP-independent mechanisms. Italian Society of Endocrinology (SIE), 1990 Thyroid cell proliferation nationallicence defined medium nationallicence thyrotropin nationallicence cyclic AMP nationallicence Damante G. Cattedra di Endocrinologia, University of Catania, 95100, Catania, Italy aut Russo D. Cattedra di Endocrinologia, University of Catania, 95100, Catania, Italy aut Foti D. Cattedra di Endocrinologia, University of Catania, 95100, Catania, Italy aut Grasso G. Cattedra di Endocrinologia, University of Catania, 95100, Catania, Italy aut Filetti S. Cattedra di Endocrinologia, University of Reggio Calabria, Via Tommaso Campanella, 88100, Catanzaro, Italy aut Journal of Endocrinological Investigation Springer International Publishing 13/5(1990-05-01), 397-402 0391-4097 13:5<397 1990 13 40618 https://doi.org/10.1007/BF03350689 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03350689 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Damante G. Cattedra di Endocrinologia, University of Catania, 95100, Catania, Italy aut NATIONALLICENCE 700 1- Russo D. Cattedra di Endocrinologia, University of Catania, 95100, Catania, Italy aut NATIONALLICENCE 700 1- Foti D. Cattedra di Endocrinologia, University of Catania, 95100, Catania, Italy aut NATIONALLICENCE 700 1- Grasso G. Cattedra di Endocrinologia, University of Catania, 95100, Catania, Italy aut NATIONALLICENCE 700 1- Filetti S. Cattedra di Endocrinologia, University of Reggio Calabria, Via Tommaso Campanella, 88100, Catanzaro, Italy aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/5(1990-05-01), 397-402 0391-4097 13:5<397 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer