caa a22 4500 465805566 CHVBK 20180323112103.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF03349520 doi (NATIONALLICENCE)springer-10.1007/BF03349520 A new approach for calcitonin determination based on target cell responsiveness [Elektronische Daten] [Mone Zaidi, T. Chambers, B. Moonga, T. Oldoni, E. Passarella, R. Soncini, I. MacIntyre] We report the development and validation of three microbioassays for calcitonin based on calcitonin-induced inhibition of the activity of isolated osteoclasts. Having precisely quantified osteoclast motility, spreading and bone resorptive activity, we have applied stringent analytical procedures to define assay characteristics. We have found that the appropriately transformed responses significantly regress on log dose of the peptides. Furthermore, potency estimates obtained using calcitonins from three species (human, salmon and a synthetic analogue of eel calcitonin) have been found to be consistent with those obtained using conventional calcitonin bioassays. In addition, the assays are remarkably sensitive (detection limit 10−15 M), highly specific and precise. We have determined plasma levels of bioactive calcitonin on samples from patients with medullary thyroid carcinoma; these are several-fold lower than those obtained using our routine calcitonin radioimmunoassay. Our study thus, forms the basis of an entirely new approach for the determination of ‘biologically active' calcitonin, and we envisage that such target cell-specific assays could become useful microanalytical methods. Italian Society of Endocrinology (SIE), 1990 Osteoclast-based nationallicence microbioassays nationallicence responsiveness nationallicence medullary thyroid carcinoma nationallicence Zaidi Mone Endocrine Unit, Department of Chemical Pathology, Royal Postgraduate Medical School, W12, London, UK aut Chambers T. Department of Histopathology, St. George's Hospital Medical School, SW17, London, UK aut Moonga B. Endocrine Unit, Department of Chemical Pathology, Royal Postgraduate Medical School, W12, London, UK aut Oldoni T. ISF, Milan, Italy aut Passarella E. ISF, Milan, Italy aut Soncini R. ISF, Milan, Italy aut MacIntyre I. Endocrine Unit, Department of Chemical Pathology, Royal Postgraduate Medical School, W12, London, UK aut Journal of Endocrinological Investigation Springer International Publishing 13/2(1990-02-01), 119-126 0391-4097 13:2<119 1990 13 40618 https://doi.org/10.1007/BF03349520 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03349520 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zaidi Mone Endocrine Unit, Department of Chemical Pathology, Royal Postgraduate Medical School, W12, London, UK aut NATIONALLICENCE 700 1- Chambers T. Department of Histopathology, St. George's Hospital Medical School, SW17, London, UK aut NATIONALLICENCE 700 1- Moonga B. Endocrine Unit, Department of Chemical Pathology, Royal Postgraduate Medical School, W12, London, UK aut NATIONALLICENCE 700 1- Oldoni T. ISF, Milan, Italy aut NATIONALLICENCE 700 1- Passarella E. ISF, Milan, Italy aut NATIONALLICENCE 700 1- Soncini R. ISF, Milan, Italy aut NATIONALLICENCE 700 1- MacIntyre I. Endocrine Unit, Department of Chemical Pathology, Royal Postgraduate Medical School, W12, London, UK aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/2(1990-02-01), 119-126 0391-4097 13:2<119 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer