caa a22 4500 465805612 CHVBK 20180323112103.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF03349515 doi (NATIONALLICENCE)springer-10.1007/BF03349515 Effect of steroid hormones and antihormones on hypothalamic beta-endorphin concentrations in intact and castrated female rats [Elektronische Daten] [Andrea Genazzani, F. Petraglia, N. Mercuri, G. Brilli, A. Genazzani, M. Bergamaschi, B. DeRamundo, A. Volpe] The aim of the present study was to evaluate the effects of estrogens and androgens on hypothalamic beta-endorphin (β-EP) concentrations. Intact or castrated female rats were chronically (2 weeks) treated with estrogen (estradiol benzoate) and/or an-tiestrogens (clomiphene, cyclophenil or epimestrol), and with androgens (dihydrote-stosterone or dehydroepiandrosterone sulphate) and/or antiandrogen (cyproterone acetate). A group of rats treated with vehicle were studied as comparison. The β-EP concentrations were measured by radioimmunoassay on acidic extracts of rat hypothalami. The administration of clomiphene and cyclophenil significantly reduced hypothalamic β-EP concentrations in intact rats, while both drugs or estradiol benzoate increased the peptide concentration in castrated rats. Both intact and castrated rats treated with epimestrol showed hypothalamic β-EP concentrations higher than vehicle treated rats. The estradiol-induced increase of β-EP was not changed by the concomitant administration of an-tiestrogens. The administration of dihydrotes-tosterone significantly decreased β-EP concentrations in both intact and castrated female rats, while the treatment with dehydroepiandrosterone sulphate only slightly decreased β-EP levels in intact female rats. The cyproterone acetate-chronically treated rats showed higher β-EP concentrations than vehicle-treated rats and these changes were reversed by the concomitant addition of dihydrotestosterone or dehydroepiandrosterone sulphate. These results showed that estrogens play a positive role while androgens negatively influence the hypothalamic β-EP concentrations in female rats, supporting the view that central β-EP might be a target of gonadal steroid feedback signals. Italian Society of Endocrinology (SIE), 1990 Estrogens nationallicence androgens nationallicence antihormones nationallicence antiestrogens nationallicence antiandrogens nationallicence beta-endorphin nationallicence hypothalamus nationallicence neuropeptides nationallicence Genazzani Andrea Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut Petraglia F. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut Mercuri N. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut Brilli G. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut Genazzani A. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut Bergamaschi M. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut DeRamundo B. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut Volpe A. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut Journal of Endocrinological Investigation Springer International Publishing 13/2(1990-02-01), 91-96 0391-4097 13:2<91 1990 13 40618 https://doi.org/10.1007/BF03349515 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03349515 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Genazzani Andrea Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Petraglia F. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Mercuri N. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Brilli G. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Genazzani A. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Bergamaschi M. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut NATIONALLICENCE 700 1- DeRamundo B. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Volpe A. Istituto Clinica Ostetrica e Ginecologica, Université di Modena, Via del Pozzo 71, 41100, Modena, Italy aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/2(1990-02-01), 91-96 0391-4097 13:2<91 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer