caa a22 4500 465805639 CHVBK 20180323112103.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF03349524 doi (NATIONALLICENCE)springer-10.1007/BF03349524 Neuroendocrine-thymus interactions. I. In vitro modulation of thymic factor secretion by thyroid hormones [Elektronische Daten] [Eugenio Mocchegiani, L. Amadio, N. Fabris] Several in vivo experimental and clinical studies suggest that the production of thymic hormones, such as thymulin (Zn-FTS), is modulated by thyroid hormones. It was not determined in these studies, however whether such modulation is exerted directly on the thymic epithelial cells which synthesize and secrete thymic hormones. In order to discriminate between direct and indirect modulation, the effect of thyroid hormones on the in vitro production of thymulin by whole thymic organ culture, as detected by the rosette inhibition assay, has been investigated. Donors of thymuses were young 6N-propyl-2 thyouracil (PTU)-treated hypothyroid Balb/c mice and normal littermates. Thymuses from hypothyroid mice were shown to produce concentrations in vitro nearly undetectable of thymic hormone, when compared to thymuses from normal mice. The in vitro addition of triiodothyronine (T3) caused a complete recovery of the thymic hormone production by thymuses from hypothyroid mice and an increased synthesis even by normal thymuses over control values. The complete blockade of in vitro thymic hormone production with cycloheximide, which inhibits mRNA and protein synthesis but not thyroid hormone permissive actions, suggests that the T3 induced increment of thymic hormone level in the supernatant is due to de novo synthesis. Furthermore, the number of thymulin-producing cells, as detected by immunofluorescence using a specific antithymulin monoclonal antibody, which is quite low in thymuses from hypothyroid mice, is completely regained after in vitro incubation with T3. These findings support the idea that the modulation of thyroid hormones on thymic endocrine activity is directly exerted at thymic level. Italian Society of Endocrinology (SIE), 1990 AZ, Azathioprine nationallicence CHX, cycloheximide nationallicence IF, immunofluorescence nationallicence PTU, 6N-propyl-2 thyouracil nationallicence T3, triiodothyronine nationallicence RFC, rosette-forming cells nationallicence GAM, goat anti-mouse nationallicence Mocchegiani Eugenio Centro di Immunologia, Istituto Nazionale Ricovero e Cura per Anziani (INRCA), 60100, Ancona, Italy aut Amadio L. Centro di Statistica, Dipartimento Ricerche Gerontologiche e Geriatn'che, Istituto Nazionale Ricovero e Cura per Anziani (INRCA), 60100, Ancona, Italy aut Fabris N. Cattedra di Immunologia, Università di Pavia, 27100, Pavia, Italy aut Journal of Endocrinological Investigation Springer International Publishing 13/2(1990-02-01), 139-147 0391-4097 13:2<139 1990 13 40618 https://doi.org/10.1007/BF03349524 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03349524 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mocchegiani Eugenio Centro di Immunologia, Istituto Nazionale Ricovero e Cura per Anziani (INRCA), 60100, Ancona, Italy aut NATIONALLICENCE 700 1- Amadio L. Centro di Statistica, Dipartimento Ricerche Gerontologiche e Geriatn'che, Istituto Nazionale Ricovero e Cura per Anziani (INRCA), 60100, Ancona, Italy aut NATIONALLICENCE 700 1- Fabris N. Cattedra di Immunologia, Università di Pavia, 27100, Pavia, Italy aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/2(1990-02-01), 139-147 0391-4097 13:2<139 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer