caa a22 4500 465805787 CHVBK 20180323112103.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF03349516 doi (NATIONALLICENCE)springer-10.1007/BF03349516 Determination of IGF-II levels in human serum using the erythroleukemia cell line K562 [Elektronische Daten] [Gian Spadoni, M. Tally, K. Florell, G. Enberg, K. Hall] A homologous radioreceptor assay (RRA) has been developed for Insulin-like Growth Factor II (IGF II) using the human erythroleukemia cell line K562. These cells have binding sites for insulin and IGF-II but not for Insulin-like Growth Factor I (IGF I). All samples were dissociated and separated from binding proteins by gel filtration at acidic pH. In healthy adults the mean serum level of radioreceptor assayable IGF II (RRA-IGF II) and 95% confidence limits were 965 ng/ml and 717-1299 ng/ml, respectively. The mean level in GH deficient patients was significantly lower (p < 0.001) compared with healthy subjects whereas no change was found in patients with acromegaly and uremia. Slightly lowered levels of RRA-IGF II were found in one patient with a tumor induced hypoglycemia. Italian Society of Endocrinology (SIE), 1990 Insulin-like Growth Factor II nationallicence acid chromatographed serum nationallicence erythroleukemia cell line K562 nationallicence radioreceptor assay nationallicence Spadoni Gian Department of Endocrinology, Karolinska Hospital, S-104 01, Stockholm, Sweden aut Tally M. Department of Endocrinology, Karolinska Hospital, S-104 01, Stockholm, Sweden aut Florell K. Department of Endocrinology, Karolinska Hospital, S-104 01, Stockholm, Sweden aut Enberg G. Department of Endocrinology, Karolinska Hospital, S-104 01, Stockholm, Sweden aut Hall K. Department of Endocrinology, Karolinska Hospital, S-104 01, Stockholm, Sweden aut Journal of Endocrinological Investigation Springer International Publishing 13/2(1990-02-01), 97-102 0391-4097 13:2<97 1990 13 40618 https://doi.org/10.1007/BF03349516 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03349516 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Spadoni Gian Department of Endocrinology, Karolinska Hospital, S-104 01, Stockholm, Sweden aut NATIONALLICENCE 700 1- Tally M. Department of Endocrinology, Karolinska Hospital, S-104 01, Stockholm, Sweden aut NATIONALLICENCE 700 1- Florell K. Department of Endocrinology, Karolinska Hospital, S-104 01, Stockholm, Sweden aut NATIONALLICENCE 700 1- Enberg G. Department of Endocrinology, Karolinska Hospital, S-104 01, Stockholm, Sweden aut NATIONALLICENCE 700 1- Hall K. Department of Endocrinology, Karolinska Hospital, S-104 01, Stockholm, Sweden aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/2(1990-02-01), 97-102 0391-4097 13:2<97 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer