caa a22 4500 46580585X CHVBK 20180323112103.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF03349656 doi (NATIONALLICENCE)springer-10.1007/BF03349656 The differentiation-inducing agent sodium butyrate produces divergent effects on albumin and thyroxine-binding globulin synthesis by human hepatoblastoma-derived (Hep G2) cells [Elektronische Daten] [L. Bartalena, F. Bogazzi, G. Donadel, E. Martino, F. Gabrielli, A. Pinchera] The addition of sodium butyrate, a differentiation-inducing agent, to the culture medium of human hepatoblastoma-derived (Hep G2) cells, produced a dose-dependent and time-dependent increase in albumin (ALB) and decrease in T4-binding globulin (TBG) synthesis and secretion. In the presence of 0.01 to 2.0 mM sodium butyrate, newly synthesized [35S]ALB progressively increased up to 139% of control cultures grown in the absence of sodium butyrate, whereas TBG synthesis was already slightly inhibited using the lowest concentrations of this agent and further diminished thereafter. The use of 5 mM and 10 mM sodium butyrate inhibited the synthesis of both proteins, probably as a consequence of toxic effects on cell cultures. The addition of 1 mM sodium butyrate for variable time intervals caused an increase in the amount of ALB recovered in the medium up to 146% after 72 h, and a decrease of TBG up to 44% of controls. These different effects on ALB and TBG occurred concomitantly with an inhibition of cell growth, as shown by the reduction in the cell number/flask compared to control cultures. At the highest sodium butyrate concentrations, a relevant impairment in the secretion of newly synthesized TBG, but not of ALB, also occurred. These divergent effects on ALB and TBG synthesis by Hep G2 cells might be related to biochemical differentiation induced by sodium butyrate in this tumoral cell system, suggesting that TBG synthesis is increased in Hep G2 cells because of their neoplastic nature. Italian Society of Endocrinology (SIE), 1990 Sodium butyrate nationallicence Hep G2 cells nationallicence TBG nationallicence hepatocellular carcinoma nationallicence tumor marker nationallicence differentiation nationallicence Bartalena L. Istituto di Endocrinologia, University of Pisa, V.le del Tirreno 64, 56018, Tirrenia-Pisa, Italy aut Bogazzi F. Istituto di Endocrinologia, University of Pisa, V.le del Tirreno 64, 56018, Tirrenia-Pisa, Italy aut Donadel G. Istituto di Chimica Biologica, University of Pisa, 56100, Pisa, Italy aut Martino E. Cattedra di Endocrinologia, University of Cagliari, 09100, Cagliari, Italy aut Gabrielli F. Istituto di Chimica Biologica, University of Pisa, 56100, Pisa, Italy aut Pinchera A. Istituto di Endocrinologia, University of Pisa, V.le del Tirreno 64, 56018, Tirrenia-Pisa, Italy aut Journal of Endocrinological Investigation Springer International Publishing 13/11(1990-12-01), 917-922 0391-4097 13:11<917 1990 13 40618 https://doi.org/10.1007/BF03349656 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03349656 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bartalena L. Istituto di Endocrinologia, University of Pisa, V.le del Tirreno 64, 56018, Tirrenia-Pisa, Italy aut NATIONALLICENCE 700 1- Bogazzi F. Istituto di Endocrinologia, University of Pisa, V.le del Tirreno 64, 56018, Tirrenia-Pisa, Italy aut NATIONALLICENCE 700 1- Donadel G. Istituto di Chimica Biologica, University of Pisa, 56100, Pisa, Italy aut NATIONALLICENCE 700 1- Martino E. Cattedra di Endocrinologia, University of Cagliari, 09100, Cagliari, Italy aut NATIONALLICENCE 700 1- Gabrielli F. Istituto di Chimica Biologica, University of Pisa, 56100, Pisa, Italy aut NATIONALLICENCE 700 1- Pinchera A. Istituto di Endocrinologia, University of Pisa, V.le del Tirreno 64, 56018, Tirrenia-Pisa, Italy aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/11(1990-12-01), 917-922 0391-4097 13:11<917 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer