caa a22 4500 465805957 CHVBK 20180323112104.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF03349648 doi (NATIONALLICENCE)springer-10.1007/BF03349648 Thyroid peroxidase in endemic goiter tissue [Elektronische Daten] [M. Sugawara, C. Summer, A. Kobayashi, S. Murakami, K. Kuma, G. Medeiros-Neto] Thyroid peroxidase (TPO) activity and TPO protein were analyzed in endemic goiter tissue under iodine deficiency and after iodine supplement. TPO was prepared from 9 endemic goiter tissues and 4 normal thyroid tissues by solubilizing enzyme with detergent. Four patients with endemic goiter received iodized oil injection 12 months before surgery. All patients had normal serum thyrotropin (TSH) and thyroid hormone levels before surgery. TPO activity was measured by iodinase assay and guaiacol assay. Endemic goiter TPO showed greater iodination activity than that of normal TPO (p<0.01). The guaiacol assay showed greater TPO activity in 6 of the 9 endemic goiter tissues than that of the normal tissue. Iodized oil treatment did not affect TPO activity or TPO proteins when compared with those in untreated endemic goiter tissues. TPO activity in endemic goiter tissue correlated with thyroid T4 5′-deiodinase activity and not with thyroid hormone content in thyroglobulin. Since thyroid T4 5′-deiodinase and TPO are under control of TSH, an increase in TPO activity in the presence of normal serum TSH may be explained by the increased sensitivity of endemic goiter tissue to TSH-one of the possible adaptation mechanisms of endemic goiter. Italian Society of Endocrinology (SIE), 1990 Thyroid peroxidase nationallicence iodine deficiency nationallicence endemic goiter nationallicence thyroid disease nationallicence thyroid hormone nationallicence thyroglobulin nationallicence Sugawara M. Endocrine Division, Medical and Research Services, Wadsworth Veterans Administration Hospital and Department of Medicine, UCLA School of Medicine, Los Angeles, Wilshire and Sawtelle Blvds, 90073, Los Angeles, California, USA aut Summer C. Endocrine Division, Medical and Research Services, Wadsworth Veterans Administration Hospital and Department of Medicine, UCLA School of Medicine, Los Angeles, Wilshire and Sawtelle Blvds, 90073, Los Angeles, California, USA aut Kobayashi A. Kuma Hospital, Kobe, Japan aut Murakami S. Endocrine Division, Medical and Research Services, Wadsworth Veterans Administration Hospital and Department of Medicine, UCLA School of Medicine, Los Angeles, Wilshire and Sawtelle Blvds, 90073, Los Angeles, California, USA aut Kuma K. Kuma Hospital, Kobe, Japan aut Medeiros-Neto G. Thyroid Laboratory, University of São Paulo, School of Medicine, Brazil aut Journal of Endocrinological Investigation Springer International Publishing 13/11(1990-12-01), 893-899 0391-4097 13:11<893 1990 13 40618 https://doi.org/10.1007/BF03349648 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03349648 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sugawara M. Endocrine Division, Medical and Research Services, Wadsworth Veterans Administration Hospital and Department of Medicine, UCLA School of Medicine, Los Angeles, Wilshire and Sawtelle Blvds, 90073, Los Angeles, California, USA aut NATIONALLICENCE 700 1- Summer C. Endocrine Division, Medical and Research Services, Wadsworth Veterans Administration Hospital and Department of Medicine, UCLA School of Medicine, Los Angeles, Wilshire and Sawtelle Blvds, 90073, Los Angeles, California, USA aut NATIONALLICENCE 700 1- Kobayashi A. Kuma Hospital, Kobe, Japan aut NATIONALLICENCE 700 1- Murakami S. Endocrine Division, Medical and Research Services, Wadsworth Veterans Administration Hospital and Department of Medicine, UCLA School of Medicine, Los Angeles, Wilshire and Sawtelle Blvds, 90073, Los Angeles, California, USA aut NATIONALLICENCE 700 1- Kuma K. Kuma Hospital, Kobe, Japan aut NATIONALLICENCE 700 1- Medeiros-Neto G. Thyroid Laboratory, University of São Paulo, School of Medicine, Brazil aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/11(1990-12-01), 893-899 0391-4097 13:11<893 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer