caa a22 4500 465805965 CHVBK 20180323112104.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF03349647 doi (NATIONALLICENCE)springer-10.1007/BF03349647 The effect of splanchnic nerve stimulation on the uptake of atrial natriuretic peptide by the adrenal gland in conscious calves [Elektronische Daten] [A. Edwards, M. Ghatei, S. Bloom] A technique has been developed with which it has been possible to quantify the output of a wide variety of agonists including catecholamines, glucocorticoids, mineralocorticoids, enkephalins and various peptides, from the adrenal gland in the conscious unrestrained calf; also to investigate responses to electrical stimulation of the peripheral end of the splanchnic nerve below any behavioural threshold. In the present study this methodology has been employed to investigate the extent to which stimulation of the splanchnic sympathetic innervation affects adrenal handling of atrial natriuretic peptide as this peptide has been identified within the adrenal medulla in this species. Stimulation of the splanchnic nerve at frequencies which raised the concentration of atrial natriuretic peptide like-immunoreactivity (ANP) by 25% led to an abrupt increase in the uptake of the peptide by the right adrenal gland by about 250%. During nerve stimulation more than 20% of the ANP that was estimated to be presented to the gland was taken up, by comparison with less than 13% of the amount presented which was taken up before and after stimulation. These results suggest that stimulation of the splanchnic nerve may specifically enhance the uptake of ANP by the adrenal gland and represent the first report of such a mechanism in respect of any biologically active peptide so far as we are aware. Italian Society of Endocrinology (SIE), 1990 Atrial natriuretic peptide nationallicence adrenal gland nationallicence calf nationallicence sympathetic nationallicence splanchnic nerve nationallicence Edwards A. Physiological Laboratory, University of Cambridge, Downing Street, CB2 3EG, Cambridge, UK aut Ghatei M. Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, W 12, London, England aut Bloom S. Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, W 12, London, England aut Journal of Endocrinological Investigation Springer International Publishing 13/11(1990-12-01), 887-892 0391-4097 13:11<887 1990 13 40618 https://doi.org/10.1007/BF03349647 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03349647 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Edwards A. Physiological Laboratory, University of Cambridge, Downing Street, CB2 3EG, Cambridge, UK aut NATIONALLICENCE 700 1- Ghatei M. Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, W 12, London, England aut NATIONALLICENCE 700 1- Bloom S. Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, W 12, London, England aut NATIONALLICENCE 773 0- Journal of Endocrinological Investigation Springer International Publishing 13/11(1990-12-01), 887-892 0391-4097 13:11<887 1990 13 40618 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer