caa a22 4500 465811248 CHVBK 20180323112116.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF02124813 doi (NATIONALLICENCE)springer-10.1007/BF02124813 Encystment of Chattonella antiqua in laboratory cultures [Elektronische Daten] [Yasuo Nakamura, Tatsushi Umemori, Masataka Watanabe, David Kulis, Donald Anderson] Cysts ofChattonella antiqua (Raphidophyceae) were obtained in laboratory cultures for the first time. They were formed on the surface of glass coverslips added to both P- and N- depleted mediums. The yield was 50-250 cysts cm−2. Crossing experiments showed the highest cyst production in mixed cultures, although cysts were observed in clonal cultures as well. Cysts had approximately double the DNA content of synchronously growing vegetative cells (G1 phase). The encystment process was also monitored: Under P-depleted conditions, ‘small cells' were formed that fused to become a ‘triangle'-shaped cell which in turn changed into a cyst. These results strongly suggest that cysts are the products of fusion and are diploid. ‘Small cells', ‘triangle'-shaped cells, and cysts are considered to be gametes, planozygotes, and hypnozygotes, respectively. Significant mortality was observed in laboratory cysts stored in the cold and dark for five months, although those remaining were capable of germination to reestablish a motile, vegetative population. Oceanographical Society of Japan, 1989 Nakamura Yasuo Laboratory of Marine Environment, the National Institute for Environmental Studies, Onogawa, Tsukuba, 305, Ibaraki, Japan aut Umemori Tatsushi College of Agriculture and Veterinary Medicine, Nihon University, Shimouma, Setagaya, 154, Tokyo, Japan aut Watanabe Masataka Laboratory of Marine Environment, the National Institute for Environmental Studies, Onogawa, Tsukuba, 305, Ibaraki, Japan aut Kulis David Biology Department, Woods Hole Oceanographic Institution, 02543, Woods Hole, MA, U.S.A. aut Anderson Donald Biology Department, Woods Hole Oceanographic Institution, 02543, Woods Hole, MA, U.S.A. aut Journal of the Oceanographical Society of Japan Kluwer Academic Publishers 46/2(1990-04-01), 35-43 0029-8131 46:2<35 1990 46 10872 https://doi.org/10.1007/BF02124813 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02124813 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Yasuo Laboratory of Marine Environment, the National Institute for Environmental Studies, Onogawa, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Umemori Tatsushi College of Agriculture and Veterinary Medicine, Nihon University, Shimouma, Setagaya, 154, Tokyo, Japan aut NATIONALLICENCE 700 1- Watanabe Masataka Laboratory of Marine Environment, the National Institute for Environmental Studies, Onogawa, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kulis David Biology Department, Woods Hole Oceanographic Institution, 02543, Woods Hole, MA, U.S.A aut NATIONALLICENCE 700 1- Anderson Donald Biology Department, Woods Hole Oceanographic Institution, 02543, Woods Hole, MA, U.S.A aut NATIONALLICENCE 773 0- Journal of the Oceanographical Society of Japan Kluwer Academic Publishers 46/2(1990-04-01), 35-43 0029-8131 46:2<35 1990 46 10872 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer