caa a22 4500 465811744 CHVBK 20180323112118.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF02374743 doi (NATIONALLICENCE)springer-10.1007/BF02374743 1,25-Dihydroxyvitamin D3 control of free cytosolic Ca2+ in the kidney [Elektronische Daten] [Yoshio Fujii, Masaharu Tsutsumi, Toshitsugu Sugimoto, Akimitsu Miyauchi, Masaaki Fukase, Takuo Fujita] Summary: Changes in cytosolic calcium ([Ca]i) have been implicated in the regulation of various cellular processes. 1,25-dihydroxyvitamin D3 (1,25 (OH)2D3) has been reported to increase [Ca]i in hepatocytes, Hl-60, parathyroid cells and osteoblasts. It is not clear, however, whether or not 1,25 (OH)2D3 increases [Ca]i in their another important target cells, renal cells. In order to adress this issue, the effect of 1,25 (OH)2D3 on [Ca]i was examined in a newly established mouse renal cortical slice perifusion system using a fluorescent Ca2+ indicator, fura-2. 1,25 (OH)2D3 at physiological concentrations increased [Ca]i within 2 min in a dose-dependent manner both in the presence and absence of extracellular Ca2+. 24,25 (OH)2D3 even at 10−7M did not affect [Ca]i levels. These results demonstrated that 1,25 (OH)2D3 very rapidly increased [Ca]i in another target cells, renal cells and our newly established mouse renal perifusion system may be useful to study the effects of vitamin D metabolites on [Ca]i in renal cells. Japanese Society of Bone Metabolism Research, 1990 Fujii Yoshio Third Division, Department of medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, 650, Kobe aut Tsutsumi Masaharu Division of Endocrinology and Bone and Mineral Metabolism, Department of Medicine, Takatsuki General Hospital, Kosobe 1-3-13, Takatsuki, 569, Osaka, Japan aut Sugimoto Toshitsugu Division of Endocrinology and Bone and Mineral Metabolism, Department of Medicine, Takatsuki General Hospital, Kosobe 1-3-13, Takatsuki, 569, Osaka, Japan aut Miyauchi Akimitsu Third Division, Department of medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, 650, Kobe aut Fukase Masaaki Third Division, Department of medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, 650, Kobe aut Fujita Takuo Third Division, Department of medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, 650, Kobe aut Journal of Bone and Mineral Metabolism Springer-Verlag 8/1(1990-04-01), 36-40 0914-8779 8:1<36 1990 8 774 https://doi.org/10.1007/BF02374743 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02374743 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fujii Yoshio Third Division, Department of medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, 650, Kobe aut NATIONALLICENCE 700 1- Tsutsumi Masaharu Division of Endocrinology and Bone and Mineral Metabolism, Department of Medicine, Takatsuki General Hospital, Kosobe 1-3-13, Takatsuki, 569, Osaka, Japan aut NATIONALLICENCE 700 1- Sugimoto Toshitsugu Division of Endocrinology and Bone and Mineral Metabolism, Department of Medicine, Takatsuki General Hospital, Kosobe 1-3-13, Takatsuki, 569, Osaka, Japan aut NATIONALLICENCE 700 1- Miyauchi Akimitsu Third Division, Department of medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, 650, Kobe aut NATIONALLICENCE 700 1- Fukase Masaaki Third Division, Department of medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, 650, Kobe aut NATIONALLICENCE 700 1- Fujita Takuo Third Division, Department of medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, 650, Kobe aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer-Verlag 8/1(1990-04-01), 36-40 0914-8779 8:1<36 1990 8 774 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer