caa a22 4500 465811817 CHVBK 20180323112118.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02377368 doi (NATIONALLICENCE)springer-10.1007/BF02377368 Mineralized phase matrix proteinases of newborn rat calvaria [Elektronische Daten] [Makoto Fukae, Takako Tanabe, Marie Yamada] Several proteinase activities associated with different molecular weight proteins were detected by substrate-gel electrophoresis using gelatin as a substrate in the 4M guanidine soluble fraction obtained from the demineralized matrix of the mineralized phase of newborn rat calvaria (mineralized phase matrix proteinases = MPM proteinases). The major gelatinase activity migrated with the molecular weight fraction of 58,000 daltons. This proteinase was active over the pH range 6-9 against gelatin. Based on the behavior against inhibitors and the differences of molecular weight, the 58kD MPM proteinase was thought to be different from the already known matrix metalloproteinases and proteinases such as kallikrain, plasmin and cathepsin B 1. Some of bone MPM proteinases may participate in the degradation of acidic proteins (sialoprotein and phosphorylated proteins) which are stained blue with Stains-all in newborn rat calvaria. Japanese Society of Bone Metabolism Research, 1990 Rat calvaria nationallicence mineralized phase nationallicence proteinase nationallicence degradation of acidic proteins nationallicence Fukae Makoto Department of Biochemistry, School of Dental Medicine Tsurumi University, 2-1-3, Tsurumi-ku, 230, Yokohama, Japan aut Tanabe Takako Department of Biochemistry, School of Dental Medicine Tsurumi University, 2-1-3, Tsurumi-ku, 230, Yokohama, Japan aut Yamada Marie Department of Biochemistry, School of Dental Medicine Tsurumi University, 2-1-3, Tsurumi-ku, 230, Yokohama, Japan aut Journal of Bone and Mineral Metabolism Springer-Verlag 8/3(1990-12-01), 12-18 0914-8779 8:3<12 1990 8 774 https://doi.org/10.1007/BF02377368 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02377368 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fukae Makoto Department of Biochemistry, School of Dental Medicine Tsurumi University, 2-1-3, Tsurumi-ku, 230, Yokohama, Japan aut NATIONALLICENCE 700 1- Tanabe Takako Department of Biochemistry, School of Dental Medicine Tsurumi University, 2-1-3, Tsurumi-ku, 230, Yokohama, Japan aut NATIONALLICENCE 700 1- Yamada Marie Department of Biochemistry, School of Dental Medicine Tsurumi University, 2-1-3, Tsurumi-ku, 230, Yokohama, Japan aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer-Verlag 8/3(1990-12-01), 12-18 0914-8779 8:3<12 1990 8 774 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer