caa a22 4500 465811825 CHVBK 20180323112118.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02377372 doi (NATIONALLICENCE)springer-10.1007/BF02377372 Insulin-like growth factor-I stimulates45Ca-accumulation in cultures of osteoblast-like cells [Elektronische Daten] [Osamu Kozawa, Kensuke Takatsuki, Haruhiko Tokuda, Masaichi Miwa, Katsuhiro Kotake, Masahiro Yoneda, Yutaka Oiso, Hidehiko Saito] Summary: The effect of insulin-like growth factor-I (IGF-I) on calcification in cloned osteoblast-like MC3T3-E1 cells was studied by measuring accumulation of45Ca into sodium dodecyl sulfate-insoluble, EDTA-extractable structure that appeared after 2 weeks of culture. The accumulation of45Ca was markedly enhanced by incubating cells with IGF-I after 6 weeks of culture. The enhancement was dose-dependent in a range between 0.1 nM and 100 nM. IGF-I stimulated alkaline phosphatase activity in the cells cultured for 7 weeks dose-dependently between 0.1 nM and 100 nM. DNA synthesis examined in the cells cultured for 7 weeks was not influenced by 100 nM IGF-I. These results suggest that IGF-I stimulates Ca-accumulation in osteoblast-like cells without stimulating their proliferation. Japanese Society of Bone Metabolism Research, 1990 Calcium nationallicence Insulin-like growth factor-I nationallicence Osteoblast nationallicence Kozawa Osamu First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut Takatsuki Kensuke First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut Tokuda Haruhiko First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut Miwa Masaichi First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut Kotake Katsuhiro First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut Yoneda Masahiro First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut Oiso Yutaka First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut Saito Hidehiko First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut Journal of Bone and Mineral Metabolism Springer-Verlag 8/3(1990-12-01), 37-40 0914-8779 8:3<37 1990 8 774 https://doi.org/10.1007/BF02377372 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02377372 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kozawa Osamu First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut NATIONALLICENCE 700 1- Takatsuki Kensuke First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut NATIONALLICENCE 700 1- Tokuda Haruhiko First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut NATIONALLICENCE 700 1- Miwa Masaichi First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut NATIONALLICENCE 700 1- Kotake Katsuhiro First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut NATIONALLICENCE 700 1- Yoneda Masahiro First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut NATIONALLICENCE 700 1- Oiso Yutaka First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut NATIONALLICENCE 700 1- Saito Hidehiko First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer-Verlag 8/3(1990-12-01), 37-40 0914-8779 8:3<37 1990 8 774 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer